Results: Characterization of Different Glial Cell Types in Culture
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Conclusion
Transcription
This protocol allows to reveal intercellular calcium signaling pattern that characterizes each cell type response in health as well as in disease. This technique allows fast and detailed biophysical characterization of complex cellular behavior. W
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We present here a protocol on how to prepare primary cultures of glial cells, astrocytes, and microglia from rat cortices for time-lapse video imaging of intracellular Ca2+ for research on pathophysiology of amyotrophic lateral sclerosis in the hSOD1G93A rat model.