Illumination and Photocrosslinking of Hydrogels via an LED Array
4:20
Setting Up and Drug Treatment of 3D, Matrix-Embedded Cultures
8:22
Results: Effect of HA Based 3D Miniaturized Cultures on Matrix Stiffness and Integrin-Binding Peptides
10:24
Conclusion
Transcription
This method enables researchers to systematically probe how matrix cues affect the phenotype of cells in 3D culture. We demonstrate the procedure using cultures of GBM cells. The main advantage of this technique is high-throughput implementation,
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The present protocol describes an experimental platform to assess the effects of mechanical and biochemical cues on chemotherapeutic responses of patient-derived glioblastoma cells in 3D matrix-mimetic cultures using a custom-made UV illumination device facilitating high-throughput photocrosslinking of hydrogels with tunable mechanical features.