Blow flies are a diverse group of flies that can be obligate or facultative parasites, as well as sapro-necrophagous. Obligate parasitic species feed exclusively on living tissues and cause myiasis in vertebrates, while sapro-necrophagous species
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Herein, two protocols for assessing food source and oviposition preferences in larvae and females of blowflies are detailed. These comprise four choices with two interacting factors: substrate type and temperature. The assays enable the determination of the food source preference of the larvae and the oviposition site preference for the females.