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Soluble basement membrane extracts are the most widely used biological matrices in cancer research, but their complex rheological behaviour makes their bioprinting difficult with commercially available bioprinting systems. This work presents a customized bioprinting strategy to produce pure matrix constructs with good shape fidelity in both single and multiple layers.
In translational research, 3D in vitro models are essential for advancing scientific understanding and therapeutic development. To this aim, Soluble Basement Membrane Extracts (SBMes), derived from mouse sarcoma cells, play the key role of replicating mechanical and biochemical features of in vivo microenvironment. They are particularly valuable in a variety of research fields, from tissue engineering to drug testing. In particular, they have become fundamental in cancer research as traditional 2D cell cultures, widely used in the field, can provide misleading information since they do not capture the three-dimensional (3D) structure and tumor microenvironment.
In cancer research, effective 3D in vitro models are crucial for better understanding cancer evolution and anticipating challenges, such as drug resistance mechanisms. Organoids have emerged as promising 3D in vitro models, offering a more accurate representation of tumor biology. They grow in SBMes as they create an environment where cells can grow and self-organize into 3D structures. However, SBMe presents significant challenges, including low mechanical properties and complex rheological behavior, preventing its use with commercially available pneumatic-extrusion bioprinting systems.
To address these limitations, we developed a low-cost, volumetric-controlled bioprinting system and a specific protocol for printing structures with SBMe. This system is based on a volumetric extruder and overcomes the limitations of traditional pneumatic-driven approaches. Once assembled and programmed with a specific g-code, the system can bioprint both pure and diluted SBMe to obtain both single and multi-layer constructs. This approach offers a more reliable and scalable method for producing 3D cell culture models, paving the way for more effective research into novel treatments and drug testing.
In translational research, creating 3D in vitro models that closely replicate the in vivo environment is essential for advancing scientific understanding and therapeutic development1. A key component in building them is the use of extracellular matrix (ECM) analogs, such as Soluble Basement Membrane Extracts (SBMes), which better reflect the complexities of natural tissues. SBMes are particularly valuable in a variety of research fields, from tissue engineering to drug testing2,3. In particular, they have become fundamental in cancer research, as they replicate mechanical and biochemica....
1. 3D printing and assembly of volumetric bioprinter
In this article, we present a protocol for bioprinting constructs made of SBMe using a low-cost, custom-made, volumetric bioprinter. We provide a detailed description and 3D printing *.STL files to build the system and we demonstrate its potential use in cancer research by printing single and multi-layer structures. Each 3D printed component has been properly cleaned from support structure or unwanted printing residues before assembling. Figure 8 shows different views of the overall assembly.......
In translational research, SBMes are particularly valuable in a variety of research fields, from tissue engineering to drug testing2,3. In particular, they have become fundamental in cancer research, as they replicate mechanical and biochemical features of the tumor microenvironment, enabling the development of models that better reflect tumor growth, metastasis, and treatment responses. SBMe is the most widely used biological matrix, especially for 3D organoids<.......
The authors have no conflicts of interest to declare.
This work was partially funded by the Accelerator Award nΒ° A26815 entitled: "Single-cell cancer evolution in the clinic" funded through a partnership between Cancer Research UK and Fondazione AIRC (nΒ° 22790) and by the European Union - Next Generation EU, Mission 4, Component 1, CUP D53D23003310006.
....Name | Company | Catalog Number | Comments |
12-multiwell plate | Sigma-Aldrich | CLS351143 | |
3D FDM printer | Flashforge | Adventurer 5M Pro | |
3D printer Motherboard | GEETECH | GT2560 REV A+ | |
6-multiwell plate | Sigma-Aldrich | M8687 | |
AC socket | HiLetgo | https://www.amazon.it/HiLetgo-Terminal-Socket -Holder-Switch/dp/B0814PT3YG/ref=sr_1_1?__ mk_it_IT=%C3%85M%C3%85%C5%BD%C3% 95%C3%91&crid=2TRIBP34VJ61A &dib=eyJ2IjoiMSJ9.zW6_VmeiudR I4yMpjJqJm7OdeoctTKW2mrEzml BAyJVa1hkM4PiJT3pLY0mXdxm1 AZQxa2_f5J4q4d5v3vUeqLkjJ6BM kWHAoeEfbEQJNuZaQ3NjmUlqWy _1AfQpnRp4VqJ2m3bkWsChztCa Ok-ZdQJlobGxJaMKg7WbV352 Hyo.0tEw2GYoPQJ4SdNYXO1XM Jrl2yXMvRey55Hj7GhISkk&dib_ tag=se&keywords=AC+socket+HiLetgo&nsdOpt OutParam=true&qid=1734702097&sprefix=ac+ socket+hiletgo%2Caps%2C173&sr=8-1 | 15 A 250 V |
Adjustable feet | EXIN DEHCEN | https://www.amazon.it/gp/product/ B0CPLTSNQB/ref=ppx_yo_dt_b_ search_asin_title?ie=UTF8&psc=1 | Height 27-40 mm |
Ball bearing 4 mm | QUARKZMAN | 684ZZ | Dimensions 4 x 9 x 4 mm |
Ball bearing 5 mm | XiKe | 625-2RS | Dimensions 5 x 16 x 5 mm |
Belt closed | Turmberg3D | https://www.amazon.it/gp/product/ B09B2FJQBV/ref=ppx_yo_dt_b_ search_asin_title?ie=UTF8&psc=1 | Width 6 mm |
Belt open | Fajoeda | https://www.amazon.it/gp/product/ B09QCTVPTH/ref=ppx_yo_dt_b_ search_asin_title?ie=UTF8&th=1 | Width 6 mm |
Bioprinting nozzle | Fisher Scientific | 17780789 | CorningΒ Standard Conical Bioprinting Nozzles |
BΓΌrker Cell Counting chamber | GLASWARENFABRIK KARL HECHT | 40443001 | |
Centrifuge | Eppendorf | 5702 R | |
Centrifuge tube | Sigma-Aldrich | CLS431470 | 15 mL |
Centrifuge tube | Sigma-Aldrich | EP0030120086 | 1.5 mL |
Display | Sigma-Aldrich | BR718905 | |
DMEMΒ | Gibco Thermofisher | 11965092 | |
FBS | Sigma-Aldrich | F7524 | |
Flanged linear ball bearing | Sourcing map | https://www.amazon.it/gp/product/ B07S6LZHM7/ref=ppx_yo_dt_b_ search_asin_title?ie=UTF8&psc=1 | LMF8UU |
Glass syringe | Fisher Scientific | 11520062 | Hamilton GasTight 1 mL (TLL terminal) |
Hinges | YASQZ | https://www.amazon.it/gp/product/ B09DNXGDGG/ref=ppx_yo_dt_b_ search_asin_title?ie=UTF8&psc=1 | |
Incubator | Fisher Scientific | 15481374 | EppendorfΒ Galaxy 48 R CO2Β Incubator, 48 L, Stainless Steel |
Inverted microscope | Nikon | Eclipse Ti2-U | |
Jumper wires | Iverntech | https://www.amazon.it/Iverntech-XH2 -54-Terminale-Stampante-Stepper/dp/ B07Q12B6K5/ref=pd_ci_mcx_mh_ mcx_views_0_image?pd_rd_w=cb2r J&content-id=amzn1.sym.57a351cf- ba07-47e8-b302-aa2aa1a3f209%3 Aamzn1.symc.ca948091-a64d-450e- 86d7-c161ca33337b&pf_rd_p=57a3 51cf-ba07-47e8-b302-aa2aa1a3f209 &pf_rd_r=YHY9RFYZJK7PYAY2RXX D&pd_rd_wg=7WkXB&pd_rd_r=8239 66cb-4977-48ce-b095-0dcedf8c372f& pd_rd_i=B07Q12B6K5&th=1 | |
LCD unit | Paradisetronic.com | https://www.amazon.it/Display-controller -conduttori-adattatore-stampante/dp/B01 DUR4064/ref=sr_1_9?__mk_it_IT=%C3 %85M%C3%85%C5%BD%C3%95%C3 %91&crid=1OAHVIIRA16X3&dib=eyJ2Ij oiMSJ9.07V2Jf7RzJjTUlIuFobUJFjUzG4 8633KkdcMXSEfnWLRX41d5vAF7xPBJ maniVxDoXecfihZzzOXlP3v-e29cUjoGLf GDyv5DoDVwp7ndohNTYvYzoi3gWkF- Hsd2YbT7tjb1Dra1afqSn26CbFdEvJ84y qyX7Gi3-7DHh8yMwdd6_7quiuW_mjsm w1nu0YQG68GZ0XM7unOsvyddQ__R_ 3dndxb3mOjgGjklEgu_KEbZJPOZDIdSo oU3nrrKHj56hCfk9ACwYpB80QGCbTPK 0p-fE0g9h02WPmtHV38UU3m5AdZsTQ HqvnVlJg703qGT-8ze8fQItw2rtbgl_J86H RMReJOhVLQBkqNwxh57CQ.yNbeFmZ ZBEUhadz2dQa8NxpZD9g4P6ighRU_97 yxgnw&dib_tag=se&keywords=display% 2B3D%2Bprinter%2Bprusa%2Bi3&nsdO ptOutParam=true&qid=1734714093&s= industrial&sprefix=display%2B3d%2B printer%2Bprusa%2Bi3%2Cindustrial% 2C143&sr=1-9&th=1 | Old Prusa i3 LCD unit. Link to commercially available alternative |
Leadscrew M8 | RS PRO | 280-408 | |
Leadscrew nut M8 | Comioke | https://www.amazon.it/dp/B0C7QB13C4? ref=ppx_yo2ov_dt_b_fed_asin_title&th=1 | Nut for M8 screws |
Leadscrew nut T8 | Aipaide | https://www.amazon.it/gp/product/B086QJCX1M/ref =ppx_yo_dt_b_search_asin_title?ie=UTF8&psc=1 | Nut for T8 screws |
Leadscrew T8 | VBESTLIFE | https://www.amazon.it/gp/product/B07CXRB52Y/ref =ppx_yo_dt_b_search_asin_title?ie=UTF8&th=1 | Length 15 mm |
L-Glutammine | Sigma-Aldrich | G7513 | |
Limit switch | CESFONJER | https://www.amazon.it/gp/product/B07SPX492J/ref =ppx_yo_dt_b_search_asin_title?ie=UTF8&psc=1 | |
Linear ball bearing (LM4UU) | A ABSOPRO | https://www.amazon.it/gp/product/B0CB3M5GHJ/ref =ppx_yo_dt_b_search_asin_title?ie=UTF8&psc=1 | Dimensions 4 x 8 x 12 mm |
Linear ball bearing (LM8LUU) | Fdit | https://www.amazon.it/LM8LUU-Linear -Motion-cuscinetti-stampante/dp/B07N 58W3GZ/ref=sr_1_1_sspa?__mk_it_IT =%C3%85M%C3%85%C5%BD%C3% 95%C3%91&crid=33VNCCCSIFJRP& dib=eyJ2IjoiMSJ9._JJ2doXI33C2JagX 8x6E2Bz2ILo10852tBi1erWqGn363ku zbcvknbMtsK6wm5jdR1P5XwC6Ovui at3kGIJPpYFOG_FiIBYT8xLkkbiphhR 4ncR6R9tPPyeN--_3RxFgKMh5tMO_ jesAcu7Anp87Qb91ruM85CKqduV-y6 HQJtAvVSXmfG4yE4N7d4_dDVKsR7 UkEmDIznJE0HIhvvI2R6vX2tMN5yMc _ZrUbUB0gPF6SgXHVJmKRizCRqL7J 64WRD-XnMSpVHY_LUVJ7gn9kJQ35 _zQw8vLD-_kRyvxwvE.4IVMdKzF1Ax mQHQhWEi6LH12UI4CmMgi288_g3zG CPY&dib_tag=se&keywords=LM8LUU+ Fdit&nsdOptOutParam=true&qid=17347 04494&s=industrial&sprefix=lm8luu+fdit+ %2Cindustrial%2C136&sr=1-1-spons&sp _csd=d2lkZ2V0TmFtZT1zcF9hdGY&psc=1 | Dimensions 8 x 15 x 45 mm |
Linear ball bearing (LM8UU) | ARCELI | https://www.amazon.it/gp/product/ B07BV3YBP2/ref=ppx_yo_dt_b_ search_asin_title?ie=UTF8&psc=1 | Dimensions 8 x 15 x 24 mm |
Magnets | OCEUMAOA | https://www.amazon.it/gp/product/ B0CB6C2RF4/ref=ppx_yo_dt_b_ search_asin_title?ie=UTF8&th=1 | 8 x 3 mm |
Matrigel | Gibco Thermofisher | LOT 0202003 | SBMe |
Motor driverΒ | Longruner | https://www.amazon.it/gp/product/ B071P41ZBW/ref=ppx_yo_dt_b_search _asin_title?ie=UTF8&psc=1 | Driver A4988 |
PBS | MicroGem, VWR | TL1006-500ML | |
Penicillin-Streptomycin | Gibco Thermofisher | 15070063 | |
Pipette tips | Sigma-Aldrich | Z740058 | BRANDΒ filter tips, racked, TipBox (volume 50-1000 ΞΌL) |
Plexiglass sheets | MVQPER | https://www.amazon.it/MVQPER-trasparenti- plexiglass-trasparente-decorazione/dp/B0CF Q4BHQZ/ref=sr_1_14?dib=eyJ2IjoiMSJ9.bf7 Mp7I5m2qAR1Fp1V6DoYF2erUlNQUK_NuF ZLz9A7uJKGa_RNeni6NiO4YQfAjVHBtZgdzI 356XVc3BIuKVpV8Pfg0rImqNXgxG-kfklAYrb rXv4tV6RL1ShFwgLzfQh8R0sEoFJPcLF2f2E EcGMlvEwOuCv6qRnS1MQ-VaA0sLPnM0ts wdL4sLVxsonWcfSrHAUrgWh4fpVNMA4Kwh 0UaeQhfexFY0dNWhPYftXvvz9LLj1uMID2E Gi_ScmYqY5LahbYOmKVVPPiPIgCVdLjaM8 yqNp2ZJGV9Fge2xa6OAHxmMeaQUqGkoQ 88x9GkX7EWuOwYO0dEUYSzHCdGONWo eZLfSJOR3C6-knjzKrOQ0ziouJfo-ktbak7v3A H53BtIOiXbT57cKqh-uc9wI_j_lHN-8okAe2yO 6upc61MoydZujuxnfLG7hwytqHQG2.ApKq8 MVs8Xihn_zcA39nML-2lpjnagGhXIx0clcJN_ E&dib_tag=se&keywords=lastra+plexiglass+ 2+mm&nsdOptOutParam=true&qid=1734702823&sr=8-14 | 2 mm thickness, transparent sheet, 21 x 32.5 cm |
Plexiglass sheets | MVQPER | https://www.amazon.it/MVQPER-trasparenti -plexiglass-trasparente-decorazione/dp/B0C FQ4BHQZ/ref=sr_1_14?dib=eyJ2IjoiMSJ9.b f7Mp7I5m2qAR1Fp1V6DoYF2erUlNQUK_N uFZLz9A7uJKGa_RNeni6NiO4YQfAjVHBtZ gdzI356XVc3BIuKVpV8Pfg0rImqNXgxG-kfk lAYrbrXv4tV6RL1ShFwgLzfQh8R0sEoFJPc LF2f2EEcGMlvEwOuCv6qRnS1MQ-VaA0s LPnM0tswdL4sLVxsonWcfSrHAUrgWh4fpV NMA4Kwh0UaeQhfexFY0dNWhPYftXvvz9 LLj1uMID2EGi_ScmYqY5LahbYOmKVVPP iPIgCVdLjaM8yqNp2ZJGV9Fge2xa6OAHx mMeaQUqGkoQ88x9GkX7EWuOwYO0dE UYSzHCdGONWoeZLfSJOR3C6-knjzKrO Q0ziouJfo-ktbak7v3AH53BtIOiXbT57cKqh- uc9wI_j_lHN-8okAe2yO6upc61MoydZujux nfLG7hwytqHQG2.ApKq8MVs8Xihn_zcA3 9nML-2lpjnagGhXIx0clcJN_E&dib_tag=se &keywords=lastra+plexiglass+2+mm&nsd OptOutParam=true&qid=1734702823&sr=8-15 | 2 transparent sheets, 2 mm thickness, 20 x 20 cm |
Plexiglass sheets | MVQPER | https://www.amazon.it/MVQPER-trasparenti -plexiglass-trasparente-decorazione/dp/B0C FQ4BHQZ/ref=sr_1_14?dib=eyJ2IjoiMSJ9. bf7Mp7I5m2qAR1Fp1V6DoYF2erUlNQUK_ NuFZLz9A7uJKGa_RNeni6NiO4YQfAjVHB tZgdzI356XVc3BIuKVpV8Pfg0rImqNXgxG- kfklAYrbrXv4tV6RL1ShFwgLzfQh8R0sEoFJ PcLF2f2EEcGMlvEwOuCv6qRnS1MQ-VaA 0sLPnM0tswdL4sLVxsonWcfSrHAUrgWh 4fpVNMA4Kwh0UaeQhfexFY0dNWhPYft Xvvz9LLj1uMID2EGi_ScmYqY5LahbYO mKVVPPiPIgCVdLjaM8yqNp2ZJGV9Fge 2xa6OAHxmMeaQUqGkoQ88x9GkX7E WuOwYO0dEUYSzHCdGONWoeZLfSJO R3C6-knjzKrOQ0ziouJfo-ktbak7v3AH53B tIOiXbT57cKqh-uc9wI_j_lHN-8okAe2yO6 upc61MoydZujuxnfLG7hwytqHQG2.ApKq 8MVs8Xihn_zcA39nML-2lpjnagGhXIx0clcJ N_E&dib_tag=se&keywords=lastra+plexigl ass+2+mm&nsdOptOutParam=true&qid=1 734702823&sr=8-16 | 2 transparent sheets, 2 mm thickness, 20 x 30 cm |
Polylactic acid (PLA) filament | Flashforge | Black 100102 | |
Power supply | SQUADO | https://www.amazon.it/SQUADO-Alimentatore -stabilizzato-dissipatore-efficienza /dp/B0DMTG6SJD/ref=sr_1_6?dib =eyJ2IjoiMSJ9.1vVze6Zt6fhKCvFb eAOSiRe57N6a1mlBnkLdHiEym4 ugHzueF6_FdOodb3sftCXb5raTg eerMMUbL5KcaI_SDa_2j7s2XohI B01QMJDyq0xQ5Gp3KLIZozDHV fGBdSrvwgyOl-BXSDy_ocZlj2TL5 e21p3K6qpvSaT5yo8WA5tngS93 Eqe8s8D6mjpnnPFrb9RpQpj299Y nNRYgr_YF3NCt3mH0r0FocKcCa LNpKtfGLCbnz_yB-360tp5SAi7A7 cgBf5I4NzQ5iQTTXAG727x0Hml mG-eaFBSJv6M1LPooZiT2aT7dx o_7tzyU6MQ9vAEozXkLTaTTMX RA_svPp96sxp93NRLReI64xpFd S3Zh1y_JPQijbMAIuvuYflhh6JlPB W6-fpW6QXl9v5wNEs_YwjzP9Sf RKxRmchzZhgsK-34XJeaEmjbRq H3piM0Zx.MfPYUNvCEhDBqLDh C-iqBEu-vjIVgsrQmOjeLuGo9YQ& dib_tag=se&keywords=power+sup ply+AC+INPUT%3A+110-220V%2 C+DC+OUTPUT%3A+12V+15A&n sdOptOutParam=true&qid=173471 4722&sr=8-6 | Old Prusa i3 power supply (AC INPUT: 110-220 V, DC OUTPUT: 12 V 15 A). Link to commercially available alternative |
Pulley 5 mm | VooGenzek | https://www.amazon.it/gp/product/ B0B8H16KWX/ref=ppx_yo_dt_b_ search_asin_title?ie=UTF8&psc=1 | Bore 5 mm, Width belt space 6 mm |
Pulley 8 mm | Yxtaii | https://www.amazon.it/gp/product/ B07X852RFN/ref=ppx_yo_dt_b_ search_asin_title?ie=UTF8&th=1 | Bore 8 mm, Width belt space 6 mm |
Ribbon cables | QUARKZMAN | https://www.amazon.it/sspa/click?ie =UTF8&spc=MTo0NDgyNTkwNzU wNjM5NzM5OjE3MzQ3MTQ1OTE6 c3BfbXRmOjMwMDEyMjk4NTMzN TYzMjo6MDo6&url=%2FQUARKZM AN-connettore-Lunghezza-Comput er-confezione%2Fdp%2FB0CQRM DQNG%2Fref%3Dsr_1_15_sspa% 3F__mk_it_IT%3D%25C3%2585M %25C3%2585%25C5%25BD%25 C3%2595%25C3%2591%26crid%3 D3BZ8Q05YA50TX%26dib%3Dey J2IjoiMSJ9.zoYnL2ViiarlDS_7165D ldxu-TTbVbWUVAP3pCybFB-bjGl wSgrpnkMhhsJj3NRo8Mvht8ev5H vGQ-UUpPom12OcOcwyRVXLAR UvMyTm8mpAdZSaOritwHRgwu2 JuW9UoS4jlCbA-gzP2FEAAH47K fFOWN6FEhc5r0YGBcSIsA6_QV6 _LA1k_ifj3c2BFm2Fj8YjW-auz7Yiy ULfZusvGX28S1K5kJdTa_YOwJY YC0Y__s-CCchHKiZgDhFjtFogUz0 Tonob4HQZiO_hkrnm3ixpNj9rgE- hvVq1FK1uD9M.kkP89x1SJ0SLxZ sv3YomYEfmkhpvP4WQf2TtC76K YAE%26dib_tag%3Dse%26keywords %3DCavo%2Bflessibile%2Ba%2Bna stro%2Bpiatto%2Bper%2Bstampant e%2B3D%2Ba%2B10%2Bpin%26n sdOptOutParam%3Dtrue%26qid% 3D1734714591%26s%3Dindustrial %26sprefix%3Dcavo%2Bflessibile %2Ba%2Bnastro%2Bpiatto%2Bper %2Bstampante%2B3d%2Ba%2B10 %2Bpin%252Cindustrial%252C170 %26sr%3D1-15-spons%26sp_csd% 3Dd2lkZ2V0TmFtZT1zcF9tdGY%26psc%3D1 | ribbon cable 2651 28AWG 10 pins |
Rigid shaft coupling | Gwolf | https://www.amazon.it/gp/product/ B088R8CW5Z/ref=ppx_yo_dt_b_ search_asin_title?ie=UTF8&psc=1 | Coupling from 5 to 8 mmΒ |
Rods 4 mm | RS-components | 682-810 | Diameter 4 mm |
Rods 5 mm | Sourcingmap | a14010200ux0214 | Diameter 5 mm |
Rods 8 mm | RS-components | 682-078 | Diameter 8 mm |
Screws | Bnnrjia | 281-984 | M2x6 - Flat head |
Screws | INCREWAY | https://www.amazon.it/INCREWAY- assortite-portatili-accessori- riparazione/dp/B07KN37M5J/ ref=sr_1_20?__mk_it_IT=%C 3%85M%C3%85%C5%BD% C3%95%C3%91&crid=12BH MAWD8SK4Z&dib=eyJ2IjoiM SJ9.SjXKoxV0NYqENPoUeS4 2-rj1Af398uiCLXDxFs0C9HfS 8vwEPmsfAMPwIMfllkAfWNY TIEU4KXhJRgRHInNI3U_aow 0EPyfufQKlA4wHz1eOJBvYO ND-5obbXfRPn7VXQ8kzBXM VfUHLAIFlJhTO8AXclkwgjB-z pRvQ6oE5Fr_1sO7lFnzURtD1 YBf65O58M3hrDXf5r_8MIz1c Asct-2P8AyWWBZAB1buYoyP gnnOiBL5EBS7twJWqcDU86m h1ywyufiUitm6kIwNuMx32D0h 0jkHna481aaYP_Q7kHgDxxrR _fFXj5gV2XqEesqGkxPCmHfF WVMqvB_YBdEAuTeL08EP_p TY50uT4UeWgdJuT58kB7Gv- Y5fPMPRS_JEQdO-oJ9DlO9n 0OszkmYwrPh-AivwVN6xh4hN 2pseXannS_on9aiNv1lh3x-f2PZ 0R.jCyNdxQiJSAsGVm7ZPhDK eFxQGBAu-M_42ZLTSSBiDg&d ib_tag=se&keywords=viti+M3x3& nsdOptOutParam=true&qid=1734 711519&sprefix=viti+m3x3%2Cap s%2C169&sr=8-20 | M2x8 - Flat head |
Screws | INCREWAY | https://www.amazon.it/INCREWAY -assortite-portatili-accessori-ripara zione/dp/B07KN37M5J/ref=sr_1_2 0?__mk_it_IT=%C3%85M%C3%85 %C5%BD%C3%95%C3%91&crid= 12BHMAWD8SK4Z&dib=eyJ2IjoiM SJ9.SjXKoxV0NYqENPoUeS42-rj1 Af398uiCLXDxFs0C9HfS8vwEPms fAMPwIMfllkAfWNYTIEU4KXhJRg RHInNI3U_aow0EPyfufQKlA4wHz 1eOJBvYOND-5obbXfRPn7VXQ8 kzBXMVfUHLAIFlJhTO8AXclkwgjB -zpRvQ6oE5Fr_1sO7lFnzURtD1YB f65O58M3hrDXf5r_8MIz1cAsct-2P8 AyWWBZAB1buYoyPgnnOiBL5EBS 7twJWqcDU86mh1ywyufiUitm6kIwN uMx32D0h0jkHna481aaYP_Q7kHg DxxrR_fFXj5gV2XqEesqGkxPCmHf FWVMqvB_YBdEAuTeL08EP_pTY50 uT4UeWgdJuT58kB7Gv-Y5fPMPRS _JEQdO-oJ9DlO9n0OszkmYwrPh- AivwVN6xh4hN2pseXannS_on9aiN v1lh3x-f2PZ0R.jCyNdxQiJSAsGVm 7ZPhDKeFxQGBAu-M_42ZLTSSB iDg&dib_tag=se&keywords=viti+M3 x3&nsdOptOutParam=true&qid=17 34711519&sprefix=viti+m3x3%2Ca ps%2C169&sr=8-20 | M2x10 - Flat head |
Screws | INCREWAY | https://www.amazon.it/INCREWAY- assortite-portatili-accessori-riparazi one/dp/B07KN37M5J/ref=sr_1_20? __mk_it_IT=%C3%85M%C3%85% C5%BD%C3%95%C3%91&crid=12 BHMAWD8SK4Z&dib=eyJ2IjoiMSJ 9.SjXKoxV0NYqENPoUeS42-rj1Af3 98uiCLXDxFs0C9HfS8vwEPmsfAM PwIMfllkAfWNYTIEU4KXhJRgRHIn NI3U_aow0EPyfufQKlA4wHz1eOJB vYOND-5obbXfRPn7VXQ8kzBXMV fUHLAIFlJhTO8AXclkwgjB-zpRvQ6 oE5Fr_1sO7lFnzURtD1YBf65O58 M3hrDXf5r_8MIz1cAsct-2P8AyWW BZAB1buYoyPgnnOiBL5EBS7twJ WqcDU86mh1ywyufiUitm6kIwNuM x32D0h0jkHna481aaYP_Q7kHgDx xrR_fFXj5gV2XqEesqGkxPCmHfF WVMqvB_YBdEAuTeL08EP_pTY5 0uT4UeWgdJuT58kB7Gv-Y5fPMPRS _JEQdO-oJ9DlO9n0OszkmYwrPh-Ai vwVN6xh4hN2pseXannS_on9aiNv1l h3x-f2PZ0R.jCyNdxQiJSAsGVm7Z PhDKeFxQGBAu-M_42ZLTSSBiDg &dib_tag=se&keywords=viti+M3x3&n sdOptOutParam=true&qid=1734711 519&sprefix=viti+m3x3%2Caps%2 C169&sr=8-20 | M3x3 - Flat head |
Screws | RS-components | 281-978 | M3x6 - Flat head |
Screws | RS-components | 281-978 | M3x10 - Flat head |
Screws | RS-components | 281-978 | M3x20 - Flat head |
Screws | Faankiton | https://www.amazon.it/Faankiton-600- M3-Incassato-Cilindrica/dp/B0CFY6V 3HV/ref=sr_1_3_sspa?crid=29NMQZ B1QM7EW&dib=eyJ2IjoiMSJ9.aiGS- zo819G6hZmghT7TFDH1TL5lzRsjT R5PBvqoHbKOBLipyX-kuevmqqPpW m_7Ngng-Wa6aLoDuXMtoqexgeNpr Qn2cISsCatqzhc8fxhJ0yIN4s3YEpD KzIroWWVNzswptkWthnGkLXwHck m4wqLr1sf0qyK9oYYlZdKo_Ypa9RQ s3aYPNd_f-DWpT5rdsG1GL3rd3b9c fLSqXxMd8QnnK3hDcg10Uz9otPwo zJC2QAQFce__CkNwDfBp5Vvgmy1 DLX4a7PolnNePrJiObgjHMJLlfwizGx fie28RAYQ.03Epc_RrVWlBUqUbHU TPDcoDUxaKBHep1CuTOhFj-fI&dib _tag=se&keywords=m3x25%2Bviti&n sdOptOutParam=true&qid=17347118 59&s=industrial&sprefix=M3x25%2Ci ndustrial%2C185&sr=1-3-spons&sp_ csd=d2lkZ2V0TmFtZT1zcF9hdGY&th=1 | M3x25 - Flat head |
Screws | Faankiton | https://www.amazon.it/Faankiton-600 -M3-Incassato-Cilindrica/dp/B0CFY6 V3HV/ref=sr_1_3_sspa?crid=29NMQ ZB1QM7EW&dib=eyJ2IjoiMSJ9.aiG S-zo819G6hZmghT7TFDH1TL5lzRsj TR5PBvqoHbKOBLipyX-kuevmqqPp Wm_7Ngng-Wa6aLoDuXMtoqexgeN prQn2cISsCatqzhc8fxhJ0yIN4s3YEp DKzIroWWVNzswptkWthnGkLXwHc km4wqLr1sf0qyK9oYYlZdKo_Ypa9R Qs3aYPNd_f-DWpT5rdsG1GL3rd3b9 cfLSqXxMd8QnnK3hDcg10Uz9otPwo zJC2QAQFce__CkNwDfBp5Vvgmy1D LX4a7PolnNePrJiObgjHMJLlfwizGxfie 28RAYQ.03Epc_RrVWlBUqUbHUTPD coDUxaKBHep1CuTOhFj-fI&dib_tag=s e&keywords=m3x25%2Bviti&nsdOptO utParam=true&qid=1734711859&s=ind ustrial&sprefix=M3x25%2Cindustrial%2 C185&sr=1-3-spons&sp_csd=d2lkZ2V 0TmFtZT1zcF9hdGY&th=1 | M3x30 - Flat head |
Screws | AERZETIX | https://www.amazon.it/AERZETIX- carbonio-fissaggio-cacciavite-mont aggio/dp/B0BDYZZ8LQ/ref=sr_1_4 ?__mk_it_IT=%C3%85M%C3%85 %C5%BD%C3%95%C3%91&crid= 217010XNB7ZXP&dib=eyJ2IjoiMSJ 9.e5QjA-q9hEvlleIzwY3ssA3x7vIBF Hpcg73tKkBJu9QbDwMwlC5L9-9u 7my7ZG5hZij0WyaijakqAoX4rIZ3rv EgvlPtN6bwKTBgqAK-3uu3GBy6K EPQ2iIEUWx_U73nonJy5IzmTDH Xijn-kRg1iCOM99S5upSWbmmazg 8lctsyXlg_tmXHjHn63WsIo7WcBuL rtVpPW8oMsZCndwh33pNQXBnk1 iStT81spopzrw3gScK1Wdz6RttH6j YL611rNBdkd-PEP2CYhDKeSKHE AqmABjHGLyG6p40k20npHqkg8g- 9Msl3fZZiS4rRxNFOHBjEdVTLBrn PjRjxTspBfZSEKQnqsGm1WPlz8cS khCs.ujWHAL4y-RNahay-1AJl34uN hUj-f6mZ7X1_KZaACvU&dib_tag=s e&keywords=m3x50%2Bviti&nsdOp tOutParam=true&qid=1734711964& s=industrial&sprefix=m3x50%2Bviti %2Cindustrial%2C136&sr=1-4&th=1 | M3x50 - Flat head |
Screws | JOCAHULFX | https://www.amazon.it/Incassato- Esagonale-Rondelle-Esagonali- Inossidabile/dp/B0DF7P5CC2/ref=sr_ 1_6?__mk_it_IT=%C3%85M%C3%8 5%C5%BD%C3%95%C3%91&crid=2 7B231M7SRJ3R&dib=eyJ2IjoiMSJ9.3 bij8nHLQAebjY8LHQS6V1GsAE07H cFGUpHAh7jA1oQIxjoTJmxjNuwg0y H14oz-YUvzD0eqVRASqAZTK2spiQ saX0pug7rWgGfENeGhztno5lMcJDT XQfe9H6ocLZANpAFv_p0dRbO6ggA vZN0TfyiuR4focFMAjr6VFGVbprEQO EaS1KRs8loF3FgL0rsmP81K806Gv5 zeSurK_sp8GEelqtm1G3J059zUdPaJ z7zJQEFPSH0TXv3lml5u9lnvnAbM0j ClF03nG5ZjB-6WUPfS41Os5DgzTpS zUTR-YZc1HnRUxy6l5grDPFmDiNZn 0Ie9MrlkcPI2A8dubQZm3d8pYVpg6Y 2OqzifmZzNsQM.Zuh5Ko4bf20Bz46c NFRHJvcyPfJyglpcvN1uM16-yOI&dib _tag=se&keywords=m4+viti+testa+sv asata&nsdOptOutParam=true&qid=17 34712054&s=industrial&sprefix=m4+v iti+testa+svasata%2Cindustrial%2C12 8&sr=1-6 | M4x8 - Flat head |
Screws | JOCAHULFX | https://www.amazon.it/Incassato-Esagonale -Rondelle-Esagonali-Inossidabile/dp/B0DF 7P5CC2/ref=sr_1_6?__mk_it_IT=%C3%85 M%C3%85%C5%BD%C3%95%C3%91&c rid=27B231M7SRJ3R&dib=eyJ2IjoiMSJ9. 3bij8nHLQAebjY8LHQS6V1GsAE07HcFG UpHAh7jA1oQIxjoTJmxjNuwg0yH14oz-YU vzD0eqVRASqAZTK2spiQsaX0pug7rWgG fENeGhztno5lMcJDTXQfe9H6ocLZANpAF v_p0dRbO6ggAvZN0TfyiuR4focFMAjr6VF GVbprEQOEaS1KRs8loF3FgL0rsmP81K8 06Gv5zeSurK_sp8GEelqtm1G3J059zUdP aJz7zJQEFPSH0TXv3lml5u9lnvnAbM0jCl F03nG5ZjB-6WUPfS41Os5DgzTpSzUTR -YZc1HnRUxy6l5grDPFmDiNZn0Ie9Mrlkc PI2A8dubQZm3d8pYVpg6Y2OqzifmZzNs QM.Zuh5Ko4bf20Bz46cNFRHJvcyPfJyglp cvN1uM16-yOI&dib_tag=se&keywords=m4 +viti+testa+svasata&nsdOptOutParam=tru e&qid=1734712054&s=industrial&sprefix= m4+viti+testa+svasata%2Cindustrial%2C1 28&sr=1-6 | M4x12 - Flat head |
Screws | Globstar | https://www.amazon.it/testa-svasata- impronta-Phillips-acciaio/dp/B083RH 5JYL/ref=sr_1_9?__mk_it_IT=%C3% 85M%C3%85%C5%BD%C3%95%C 3%91&crid=XEVGIE9R1K2F&dib=ey J2IjoiMSJ9.sYNfljBDPU1N2px8nueT zi6D61Z46HD98MSuIwqXNKrfkSOM GYgywNTC8-dsYqNXiZEMS42kGB4 QdBtz_TVFLvgLCFQVl9W5n7Bo4fQS _VKHh9VLmA_RjdF75wPsSbRPIdSA nidi0pehfkRoBIrEtYVn-cUq4TXH0P1D pGLc_4J88tU02ieLVee-YarMxZLKeLYt kK6B2CmM6d_AE0K7UJofwnOCOLp 3R72KpMTltjkJutf0WxBFsNQhYG8Wz 0q1o_3uRs_aNM0wJtGeSohS3fABN8C z-kYxdfOikF1zi4nE1wn8YUw8lfsCxeUI ioUp4Lz0GaXhHkkXxC5jssOsuOyW_6 CeDYrx0AOAy1URHBY.4xc_PNzUrZe FFz0FV9hV7uu_-Es6f3jhi6koImPVcgw &dib_tag=se&keywords=m4x70%2Bvi ti%2Btesta%2Bsvasata&nsdOptOutPa ram=true&qid=1734712182&s=industri al&sprefix=m4x70%2Bviti%2Btesta%2 Bsvasata%2Cindustrial%2C134&sr=1-9&th=1 | M4x70 - Flat head |
Screws | Aerzetix | https://www.amazon.it/AERZETIX-cilin drica-impronta-intaglio-montaggio/dp/B 0BLNJ9PTX/ref=sr_1_8?__mk_it_IT=% C3%85M%C3%85%C5%BD%C3%95 %C3%91&crid=3AESCF6VKG6B6&di b=eyJ2IjoiMSJ9.3HlweLtFpKk8huuM4 tfcAbDSl2biH6iWGO7PXpXKxzQnVM cKVrvCI0CkZanzuaduqvaeCs8DLxlFs c_s4-qvSDCE8dvXDhuO4nHPwIhPAl BnL5G6tLMycq0ZS0ZN83GAj_VOUc GVLq4fdcfeurabYdPzrWf3PpwM6_-y OlEOLVvlzzBKgQWO7fiMSCFuGA6 X4j8xVlNzobgrSfsnDNqZpEgH289rp AslvAA9D2YEmvJSm0CRqWecsKPJ iB8yNhelz01McT-qy3s6utg4_Thec18s iTwIY2bFdQ5XYhkTzBxAF86lr6VMXZ 3Ym26ZiedQE1_7ceB7jYcyQ5mOMR fx0d8pYVpg6Y2OqzifmZzNsQM._rEh uS8xCOyhS7xqQQe9H3kwn-ppl22Rf VmzFpFpyW0&dib_tag=se&keyword s=m4x80%2Bviti%2Btesta%2Bsvasat a&nsdOptOutParam=true&qid=17347 12214&s=industrial&sprefix=m4x80% 2Bviti%2Btesta%2Bsvasata%2Cindu strial%2C141&sr=1-8&th=1 | M4x80 - Flat head |
Screws | Globstar | https://www.amazon.it/testa-svasata- impronta-Phillips-acciaio/dp/B083RJ S9NT/ref=sr_1_30?__mk_it_IT=%C3 %85M%C3%85%C5%BD%C3%95% C3%91&crid=15MBBIPYCF06S&dib =eyJ2IjoiMSJ9.wHpVBrZH8GsdSIko N0T0-2663KN2Tr-H4orSaQtmYf64T nTN15zshBEbfwlrPNyJaD4F1cXZvO Evle2B5qQb_Un-tR8WzVwAxQFWe SMPaYKjM5ZgpbhFaaS6Zj0BQntEIA hyTnvnLg4IBEE9y-4jQ66ZIkmXcWFo zXK-eTFnle6uFI4nXhbCXTWjM5MP_ sNolDP0O4HS7Bnxk_wanuwC9zEuH ZeVNsejl6gwYR0IlJK5iu0V9Y-bgFcr1- iTBhrARDKG7-0Nc5188TSaN9snT7K 6yO2J3ctfC24PTGnaZAFYKMvrNqz OAihVBKIW8xOhIx0ZRipPa9ggsMs _pe1VgY2dCXtUrE_bo1dd4oGlUuw .v5iiMZ2rMQlZlZs2WDyqM_mo1utvu PD71Jyb-O7BY1Y&dib_tag=se&key words=m5x8+viti+testa+svasata&ns dOptOutParam=true&qid=17347123 79&s=industrial&sprefix=m5x8+viti+ testa+svasata%2Cindustrial%2C201 &sr=1-30 | M5x8 - Flat head |
Screws | Globstar | https://www.amazon.it/testa-svasata- impronta-Phillips-acciaio/dp/B083RH M4BY/ref=sr_1_30?__mk_it_IT=%C3 %85M%C3%85%C5%BD%C3%95% C3%91&crid=15MBBIPYCF06S&dib= eyJ2IjoiMSJ9.wHpVBrZH8GsdSIkoN 0T0-2663KN2Tr-H4orSaQtmYf64Tn TN15zshBEbfwlrPNyJaD4F1cXZvO Evle2B5qQb_Un-tR8WzVwAxQFW eSMPaYKjM5ZgpbhFaaS6Zj0BQnt EIAhyTnvnLg4IBEE9y-4jQ66ZIkmX cWFozXK-eTFnle6uFI4nXhbCXTW jM5MP_sNolDP0O4HS7Bnxk_wan uwC9zEuHZeVNsejl6gwYR0IlJK5iu 0V9Y-bgFcr1-iTBhrARDKG7-0Nc5 188TSaN9snT7K6yO2J3ctfC24PTG naZAFYKMvrNqzOAihVBKIW8xOhI x0ZRipPa9ggsMs_pe1VgY2dCXtUr E_bo1dd4oGlUuw.v5iiMZ2rMQlZlZs 2WDyqM_mo1utvuPD71Jyb-O7BY 1Y&dib_tag=se&keywords=m5x8% 2Bviti%2Btesta%2Bsvasata&nsdOpt OutParam=true&qid=1734712379&s =industrial&sprefix=m5x8%2Bviti%2 Btesta%2Bsvasata%2Cindustrial%2 C201&sr=1-30&th=1 | M5x10 - Flat head |
Screws | Globstar | https://www.amazon.it/testa-svasata- impronta-Phillips-acciaio/dp/B083RH 9SYZ/ref=sr_1_30?__mk_it_IT=%C3 %85M%C3%85%C5%BD%C3%95% C3%91&crid=15MBBIPYCF06S&dib =eyJ2IjoiMSJ9.wHpVBrZH8GsdSIko N0T0-2663KN2Tr-H4orSaQtmYf64T nTN15zshBEbfwlrPNyJaD4F1cXZv OEvle2B5qQb_Un-tR8WzVwAxQF WeSMPaYKjM5ZgpbhFaaS6Zj0BQ ntEIAhyTnvnLg4IBEE9y-4jQ66ZIkm XcWFozXK-eTFnle6uFI4nXhbCXT WjM5MP_sNolDP0O4HS7Bnxk_w anuwC9zEuHZeVNsejl6gwYR0IlJK5 iu0V9Y-bgFcr1-iTBhrARDKG7-0Nc5 188TSaN9snT7K6yO2J3ctfC24PTG naZAFYKMvrNqzOAihVBKIW8xOhIx 0ZRipPa9ggsMs_pe1VgY2dCXtUrE _bo1dd4oGlUuw.v5iiMZ2rMQlZlZs2 WDyqM_mo1utvuPD71Jyb-O7BY1Y &dib_tag=se&keywords=m5x8%2Bvi ti%2Btesta%2Bsvasata&nsdOptOutP aram=true&qid=1734712379&s=indus trial&sprefix=m5x8%2Bviti%2Btesta% 2Bsvasata%2Cindustrial%2C201&sr= 1-30&th=1 | M6x20 - Flat head |
SD card | SanDisk | https://www.amazon.it/SanDisk-Ultra- Scheda-memoria-classe/dp/B08GYG6 T12/ref=sr_1_8?crid=3ETH6HJL8VKD A&dib=eyJ2IjoiMSJ9.5omqicizrizfHo8r pPQ6sWJ_C0sfwUqPQv6VrziwK-SCX 4-aSgFGois1pfvTwB0SuDxdNjByo2zU fBonWwUKAf3clazXEIGNl0GhojQmvj Bt_6RS31QcAM5Y50BpAdndMXIHy9 iGTswgYEwnbt9uj56G_soC6OdPiTvr W3M8jwtTxQDUl8UcHZeRMPq0T8sd v4hskHGmipFItAzE1lyDiy0otrqUhz-ph OggXBAM6gMyGf0qBtU1AFwfj81Shl HS2qojCiRq_JLKyBkc_Y4P_V1YtsGc MxnoG6YzYowwEYbX7uTFWJB-Wvc B_vYw-D5s0B-HDSYRSl-C60yhtqhrUw grg9w6PN5U5s01vos4bauIgIN6lOez1 tz2OHwL5YL3QCHKxNnJV0IM42cH0 du8fIEIbUwYC5jRLqowx1UyaQKQ4W WaGozdZ9l5qUoeQ7KU.iOPo2BYLK STjqjB8BevaVgPa5QD7BuP9CieN6u 4_Qeo&dib_tag=se&keywords=sd% 2Bcard%2Bsandisk&nsdOptOutParam =true&qid=1734704871&sprefix=SD% 2Bcard%2Bsan%2Caps%2C141&sr=8-8&th=1 | SanDisk 32 GB Extreme PRO SD card, UHS-I |
Self-aligning Flange bearing | Brateuanoii | KFL08 | 8 mm inner diameter |
Slicing software | Ultimaker Cura | https://ultimaker.com/it/software/ ultimaker-cura/?gad_source=1& gclid=CjwKCAiAyJS7BhBiEiwAy S9uNUjnbkNIYNUco6YaZYHcFT 2tqBac23MU4Qx3FaMXjl_UifJ_ k8as3BoCeVkQAvD_BwE | Ultimaker Cura 4.9.0 |
Springs | YIXISI | https://www.amazon.it/sspa/click?ie =UTF8&spc=MTo4NDAzNDQ1MzM 0OTUyODQzOjE3MzQ3MDQ4MD c6c3BfYXRmOjMwMDM5MDQzMz kzODAzMjo6MDo6&url=%2FYIXIS I-Assortimento-Estensione-Compre ssione-Dispositivi%2Fdp%2FB0DD T3KTBF%2Fref%3Dsr_1_1_sspa% 3F__mk_it_IT%3D%25C3%2585M %25C3%2585%25C5%25BD%25 C3%2595%25C3%2591%26crid% 3D1NT7CA1YJGWK4%26dib%3D eyJ2IjoiMSJ9.IhFmevq0fXTGGwE cGvfUxxLOudQqQcPjSGXlWklXO HY2Ahr2XxWSg6MaNBgZ71N1pQ h9Wkp5c2ary4Hm2xVi4dTH8Ny4 4-OhlcrTn0G8emvX66Zu7U0LGm xGYKyKCGxzlhwYC4EnlmTSH_O JnA5oG-BQzuxFBdCrce0txErCEm S40xONaaYp2R5yMiPDw2PqsPP jLmoSCYs4XctxEvAwn_5rp71t0U QsZNku6-jDiiuh5klpC9DsnjipLFP unRCbITWnw6wslENLdJtPDOGF 8vbO5WFf7PnUy4BZ7tUPt7I.B6j eUKwx1_t0sPaxmSHYVbRd1MG w-kosEYkHuKlPtpQ%26dib_tag% 3Dse%26keywords%3Dmolle%2B YIXISI%26nsdOptOutParam%3Dtr ue%26qid%3D1734704807%26spr efix%3Dmolle%2Byixisi%252Caps %252C137%26sr%3D8-1-spons%2 6sp_csd%3Dd2lkZ2V0TmFtZT1zcF 9hdGY%26psc%3D1 | |
Stepper motor | Twotrees 1234 | 17HS4401 | Stepper motor bipolar NEMA 17 42 Ncm 1.5 A |
Stepper motor | STEPPERONLINE | UL1007AWG6 | Stepper motor bipolar NEMA 17 42 Ncm 1.5 A |
Stepper motor fixing bracket | YOTINO | https://www.amazon.it/gp/product/ B07D7XH97T/ref=ppx_yo_dt_b_ search_asin_title?ie=UTF8&psc=1 | |
Threaded nut M2 | RS-components | 281-984 | |
Threaded nut M3 | RS-components | 281-984 | |
Threaded nut M4 | RS-components | 281-984 | |
Threaded nut M5 | RS-components | 281-984 | |
Threaded nut M6 | RS-components | 281-984 | |
TRAMP - C1 cell line | ATCC | CRL-2730 | Epithelial cell line isolated from the prostate of an adult male transgenic mouse with adenocarcinoma |
Trypan blue | Gibco Thermofisher | 15250061 | |
Trypsin | Gibco Thermofisher | 15400054 | Sterile |
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