Ticks are hematophagous ectoparasites, Rhipicephalus microplus, popularly known as the cattle tick has enormous negative economical impact on livestock in tropical areas. In addition, increasing concerns about degradated environmental conditions a
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Analysis of tick hemolymph represents an important source of information on how some pathogens cause disease and how ticks immunologically respond to this infection. The present study demonstrates how to inoculate fungal propagules and collect hemolymph from Rhipicephalus microplus engorged females.