Viral Transgene Expression in Rodent Ventricular Tissues
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Assessment of Cardiac Arrhythmia Susceptibility
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Results: Assessment of Cardiac Susceptibility to Arrhythmias
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Conclusion
Trascrizione
This protocol allows the user to achieve efficient transgene expression in the ventricular myocardium, facilitating gene expression studies to examine cardiac arrhythmias and function in the ventricular tissue. The technique for viral transgene ex
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The present protocol describes methods for transgene expression in rat and mouse hearts by direct intramyocardial injection of the virus under echocardiography guidance. Methods for the assessment of the susceptibility of hearts to ventricular arrhythmias by the programmed electrical stimulation of isolated, Langendorff-perfused hearts are also explained here.