Induction of Adoptive DTH and Measuring Inflammation in the Ear
2:54
Removing Dermal Layers from Ear
5:27
Preparing Ear for 2-photon Imaging
7:48
Imaging the T cell Motility in the Ear
9:26
Conclusion
Here we demonstrate a method for inducing and recording the progress of a delayed type-hypersensitivity (DTH) reaction in the rat ear. This is followed by a demonstration of the preparation of rat ear tissue for two-photon imaging of the effector / memory T cell response.