Detection of Vitronectin (Vn) Binding at the Bacterial Surface Using Flow Cytometry
2:18
ELISA Analysis of the Interaction Between Recombinant Protein H (PH) and Vn
4:11
Study of the Interaction Kinetics of Recombinant PH and Vn Using Bio-layer Interferometry
4:33
Bacterial Adherence to the Vn-coated Glass Surfaces
5:32
Vn-dependent Adherence of Bacteria to Epithelial Cells
7:29
Analysis of Vn-dependent Resistance to the Bactericidal Activity of Human Serum
8:29
Results: Interaction of Vitronectin and Bacteria
10:10
Conclusion
文字起こし
This method can help answer key questions in host-pathogen interaction field such as how bacterial pathogens cause disease in humans by utilizing human proteins. The main advantage of this technique is it can answer if any pathogen can utilize Vit
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This report describes protocols for characterizing interactions between bacterial outer membrane proteins and the human complement regulator vitronectin. The protocols can be used to study the binding reactions and biological function of vitronectin in any bacterial species.