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Abstract

Medicine

A Simple Cuff Technique for Murine Left Lung Transplantation

Published: January 26th, 2024

DOI:

10.3791/65979

1Department of Surgery, University of Maryland Medical Center, 2Division of Allergy, Asthma and Clinical Immunology, Mayo Clinic, 3Department of Surgery, Washington University in St. Louis, 4Department of Pathology & Immunology, Washington University in St. Louis, 5Department of Microbiology and Immunology, University of Maryland Medical Center

Abstract

Over the past decade, our laboratory has made significant progress in developing and refining vascularized mouse lung transplantation models using an efficient and highly reliable "cuff technique" of transplantation. This article describes a sophisticated and comprehensive method for orthotopic lung transplantation in a vascularized orthotopic lung model, representing the most physiologic and clinically relevant model of mouse lung transplantation to date. The transplantation process consists of two distinct stages: donor harvest and subsequent implantation into the recipient. The method has been successfully mastered, and with several months of sufficient training, a skilled practitioner can perform the procedure in approximately 90 min from skin-to-skin. Surprisingly, once individuals overcome the initial learning curve, the survival rate during the perioperative period approaches nearly 100%. The mouse model allows for the use of multiple commercially available transgenic and mutant strains of mice, enabling the study of tolerance and rejection. Additionally, the unique features of this model make it a valuable tool for investigating tumor biology and immunology.

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Keywords Mouse Lung Transplantation

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