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Author Spotlight: Optimizing Apoplast Protein Extraction for Efficient Recovery of Recombinant Proteins from Plant Cells

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01:02 min

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July 5th, 2024

DOI :

10.3791/66852-v

July 5th, 2024

•

610 Views

Huiying Ji¹^,², Tianhao Zhang², Han Li²^,³, Chunyu Wang¹^,², Rongxiang Fang², Lili Zhang¹^,²^,⁴, Yan Huo²

¹Shandong Academy of Medical Sciences, Shandong First Medical University, ²State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, ³College of Veterinary Medicine, Shanxi Agricultural University, ⁴Beijing Life Science Academy

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Eukaryotic Expression System

Green Fluorescent Protein

Nicotiana Benthamiana

Infiltration centrifugation Method

Nickel Affinity Chromatography

Intracellular Proteins

Glycosylation

Protein Homogeneity

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