Dissection and Preparation of Mouse Seminiferous Tubules
3:16
Squashing and Labeling Prepared Seminiferous Tubules
5:47
Analysis and Imaging Tubule Squash Preparations
7:03
Results: Visualization of Meiotic Progression Using the Tubule Squash Preparation
8:35
Conclusion
필기록
The overall goal of this seminiferous tubule squash technique is to visualize the cytological features of mouse spermatogonia, spermatocytes, and spermatids while preserving their cellular integrity. For this demonstration, we focus on the analysi
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The goal of this tubule squash technique is to rapidly assess cytological features of developing mouse spermatocytes while preserving cellular integrity. This method allows for the study of all stages of spermatogenesis, and can be easily implemented alongside other biochemical and molecular biological approaches for the study of mouse meiosis.