CD34+ Hematopoietic Stem and Progenitor Cell (HSPC) Gene Modification and Overnight Recovery (Day 0)
6:33
Cell Harvest and Infusion Preparation (Day 1)
7:42
Liquid Culture Quality Control (Days 1-5)
9:19
Results: Representative HSPC Identification and Analysis
10:34
Conclusion
필기록
To evaluate the efficiency and safety of promising and novel gene therapy approaches, the comprehensive testing of gene-modified cells in validated preclinical in vivo models is crucial. The conservation of relevant cell surface markers'cross-reac
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The goal of this protocol is to isolate nonhuman primate CD34+ cells from primed bone marrow, to gene-modify these cells with lentiviral vectors, and to prepare a product for infusion into the autologous host. The total protocol length is approximately 48 h.