Induction of Spawning, In Vitro Fertilization, and De-jellying
1:48
Surgical Removal of an Aboral Tissue from a Gastrula Embryo
3:38
Genomic DNA Extraction and Genotyping PCR
4:54
Results: Genotyping Results of Sea Anemone Embryos
6:07
Conclusion
필기록
This protocol enables a researcher to identify mutant sea anemones during early embryogenesis so that post-embryonic developmental phenotype can be analyzed. The main advantage of this technique is that it can be used to genotype individual sea an
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The goal of this protocol is to genotype the sea anemone Nematostella vectensis during gastrulation without sacrificing the embryo.