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JoVE Journal

Biochemistry

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Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress

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10:57 min

October 13th, 2019

October 13th, 2019

7,307 Views

0:04

Title

1:08

Coating Cell Culture Plates with the Extracellular Matrix Protein Fibronectin

2:13

Preparing Anchorage-dependent Cells for the Adhesion Assay

5:28

Cell Fixation and Antibody Staining for Immunofluorescence

7:41

Results: Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress

9:43

Conclusion

필기록

This method will enable researchers to quantify the early dynamics of cellular adhesion and spreading of anchorage-dependent cells onto the extracellular matrix protein fibronectin during oxidative stress. This technique is versatile and can be ad

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This method is useful for quantifying the early dynamics of cellular adhesion and spreading of anchorage-dependent cells onto the fibronectin. Furthermore, this assay can be used to investigate the effects of altered redox homeostasis on cell spreading and/or cell adhesion-related intracellular signaling pathways.

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