The protocol described in this video will define the experimental procedures required for the life-size imaging of spreading cells and the use of a computational tool that quantifies cells spreading dynamics in an unbiased and automated fashion. T
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In this protocol, we present the experimental procedures of a cell spreading assay that is based on live-cell microscopy. We provide an open-source computational tool for the unbiased segmentation of fluorescently labeled cells and quantitative analysis of lamellipodia dynamics during cell spreading.