Isolation and Selection of Potential Entomopathogenic Fungi (EPF)
2:27
1st Pathogenicity Screen and 2nd Virulence Test of Fungal Isolates
3:42
Molecular and Morphological Identification of Potential Entomopathogenic Fungi (EPF)
5:23
Investigation of Conidial Productivity and Thermotolerance
7:07
Effective Conidia Number (ECN) Ranking
7:31
Results: Thermotolerance and Conidial Production Data into the ECN Formula and PCA Analysis
8:40
Conclusion
필기록
The method for entomopathogenic fungi isolation is important for developing microbial control agents. This protocol can be used to isolate and select high virulence entomopathogenic fungal isolates from the soil samples. This technique includes th
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Here we present a protocol based on the mealworm (Tenebrio molitor)-bait system that was used for isolating and selecting entomopathogenic fungi (EPF) from soil samples. An effective conidia number (ECN) formula is used to select high stress tolerant EPF based on physiological characteristics for pest microbial control in the field.