Infusion Set Up and System Settings of the Microinjection Controller
5:37
Infusion of SCFAs into Lateral Ventricle and Validation of Intracerebroventricular Injection
6:29
Results: Analysis of SCFAs-Infused Mice
7:09
Conclusion
필기록
The metabolites released by gut microbiota produce complex effects on the host. This protocol presents a method to interrogate the role of gut metabolites in the mouse brain and behavior. This technique provides a precise, meticulous, and validate
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Gut-derived microbial metabolites have multifaceted effects leading to complex behavior in animals. We aim to provide a step-by-step method to delineate the effects of gut-derived microbial metabolites in the brain via intracerebroventricular delivery via a guide cannula.