Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

Podsumowanie

A protocol for the elucidation of plant thylakoid protein complex organization and composition with blue native polyacrylamide gel electrophoresis (BN-PAGE) and 2D-SDS-PAGE is described. The protocol is optimized for Arabidopsis thaliana, but can be used for other plant species with minor modifications.

Streszczenie

Photosynthetic electron transfer chain (ETC) converts solar energy to chemical energy in the form of NADPH and ATP. Four large protein complexes embedded in the thylakoid membrane harvest solar energy to drive electrons from water to NADP⁺ via two photosystems, and use the created proton gradient for production of ATP. Photosystem PSII, PSI, cytochrome b₆f (Cyt b₆f) and ATPase are all multiprotein complexes with distinct orientation and dynamics in the thylakoid membrane. Valuable information about the composition and interactions of the protein complexes in the thylakoid membrane can be obtained by solubilizing the complexes from the membrane integrity by mild detergents followed by native gel electrophoretic separation of the complexes. Blue native polyacrylamide gel electrophoresis (BN-PAGE) is an analytical method used for the separation of protein complexes in their native and functional form. The method can be used for protein complex purification for more detailed structural analysis, but it also provides a tool to dissect the dynamic interactions between the protein complexes. The method was developed for the analysis of mitochondrial respiratory protein complexes, but has since been optimized and improved for the dissection of the thylakoid protein complexes. Here, we provide a detailed up-to-date protocol for analysis of labile photosynthetic protein complexes and their interactions in Arabidopsis thaliana.

Wprowadzenie

Large multisubunit protein complexes photosystem PSI and PSII, Cyt b₆f and ATPase coordinate the production of NADPH and ATP in photosynthetic light reactions. In higher plant chloroplasts, the complexes are located in the thylakoid membrane, which is a structurally heterogeneous membrane structure, comprising appressed grana and non-appressed stroma thylakoids. Blue native polyacrylamide gel electrophoresis (BN-PAGE) is an extensively used method in the analysis of large multisubunit protein complexes in their native and biologically active form. The method was established for the dissection of mitochondrial membrane protein complexes¹, but has later been customized for the separation of protein complexes from the thylakoid membrane network³. The method is suitable (i) for the purification of individual thylakoid protein complexes for structural analysis, (ii) for determining native interactions between protein complexes and (iii) for the analysis of overall organization of the protein complexes upon changing environmental cues.

Prior to the separation, protein complexes are isolated from the membrane with carefully chosen nonionic detergents, which are generally mild and preserve the native structure of the protein complexes. Detergents contain hydrophobic and hydrophilic sites and form stable micelles above a certain concentration, called a critical micellar concentration (CMC). Increasing the detergent concentration above the CMC results in disruption of the lipid-lipid interactions and in the solubilization of protein complexes. The choice of detergent depends on the stability of the protein complex of interest and on the solubilization capacity of the detergent. Routinely used detergents include α/β-dodecyl-maltoside and digitonin. Following the solubilization of protein complexes in their native state, insoluble material is removed by centrifugation. In higher plants, the thylakoid membrane is highly heterogenic in structure and some detergents (e.g., digitonin) selectively solubilize only a specific fraction of the membrane³. Therefore, to characterize the protein complex organization or the interactions between the protein complexes, it is crucial to always determine the solubilization capacity of the chosen detergent by determining the chlorophyll content and the chlorophyll a/b ratio of supernatant to assess the yield and the represented thylakoid (sub)domain, respectively, of the solubilized fraction. The chlorophyll a/b ratio in intact thylakoids of growth-light acclimated plants is typically around 3, whereas the chl a/b value of thylakoid fractions enriched either in grana or stroma thylakoids falls below (~2.5) or exceeds (~4.5) the value of the total thylakoids, respectively.

To provide negative charge to the protein complexes, Coomassie brilliant blue (CBB) dye is added to the solubilized sample. Due to the charge shift, protein complexes migrate towards the anode and are separated on an acrylamide (AA) gradient according to their molecular mass and shape. Effective and high-resolution separation is achieved by using a linear acrylamide concentration gradient. During the electrophoresis, the protein complexes migrate towards the anode until they reach their size-dependent pore-size limit. The pore-size of polyacrylamide gel depends on (i) the total acrylamide/bis-acrylamide concentration (T) and (ii) on the cross-linker bis-acrylamide monomer concentration (C) relative to the total monomers⁴. After the separation with BN-PAGE, the protein complexes can be further subdivided into their individual protein subunits by second-dimension (2D)-SDS-PAGE. Here, we describe a detailed protocol for the analysis of thylakoid membrane protein complexes by BN-PAGE/2D-SDS-PAGE.

Protokół

1. Preparing BN Gel^1,2,3

1. Set up the gel caster with 8 cm x 10 cm plates (rectangular glass and notched alumina plate) according to manufacturer's instructions using 0.75 mm spacers.
2. Place a gradient mixer on a stir plate and connect it with the peristaltic pump by a tubing. Attach a syringe needle to the other end of the tubing and place the needle between the glass and aluminum plate. Place magnetic stirrer to the "heavy" (H)-chamber.
3. Prepare the 3.5% (v/v) and 12.5% (v/v) acrylamide (AA) solutions in 15 mL conical centrifuge tubes for the separation gel gradient (see recipes in Table 1). To prevent untimely polymerization, keep the centrifuge tubes on ice while preparing the solutions.
   CAUTION: Acrylamide is neurotoxic and carcinogenic, wear protective clothes and gloves.
4. Add 5% APS and TEMED right before pipetting the solutions to the gradient mixer. Pipet the 12.5% solution to the H-chamber.
   1. Remove air bubbles from the channel connecting the "light" (L) and H-chamber by opening the valve connecting the two chambers allowing solution to enter to the L-chamber. Close the valve and pipet the traces of solution back to H-chamber.
   2. Finally, pipet the 3.5% solution to the L-chamber.
5. Switch on the magnetic stirrer (the speed of the stir is not critical, but it should ensure proper mixing of the heavy and light solutions), open the valves and switch on the peristaltic pump. Allow the gel solutions to flow between the glass and aluminum plate, the flowrate should be roughly 0.5 mL/min. The needle must be above the liquid all the time, it can be attached to the upper part of the glass plate with a tape.
6. When the H- and L-chambers have emptied, fill them with ultrapure water and allow water to gently overlay the gel surface. The gel polymerization takes around 1-2 hours at RT.
7. Prepare the 3% acrylamide solution (see recipe in Table 1) for the stacking gel at RT. Pipet the stacking gel on top of the polymerized separation gel (before casting the stacking gel, remove the water overlaying the gel surface) and place a sample gel comb between the glass and aluminum plate avoiding air bubbles.
   1. Allow to polymerize 30-60 min at RT. Remove the comb gently under ultrapure water. Store the gel at +4 ˚C.
      Pause point. The gel can be stored at +4 ˚C for a few days. The gel should be kept in moist conditions to avoid drying of the wells and the gel surface.

2. Thylakoid Solubilization^1,2,3

NOTE: All steps should be performed under very dim light. Keep samples and buffers on ice.

1. Dilute isolated thylakoids with ice-cold 25BTH20G buffer to a final chlorophyll concentration of 1 mg/mL. For 2D-BN-SDS-PAGE analysis roughly 4-8 µg of chlorophyll/ sample is suitable.
   NOTE: The thylakoids used in the experiments must be isolated from fresh leaves (for protocol of thylakoid isolation, see³)
2. Add an equal volume of detergent buffer, i.e., 2% β-DM (w/v) or 2% digitonin (w/v). Mix the detergent to the thylakoid sample gently with the pipet tip and avoid making air bubbles. The final concentration of the detergent is 1% and that of the thylakoids 0.5 mg Chl/mL.
   1. Solubilize the thylakoids for 2 min on ice (β-DM) or 10 minutes at RT with continuous gentle mixing on a rocker/shaker (digitonin).
      NOTE: Digitonin and β-DM are generally used for the solubilization of thylakoid protein complexes. If other non-ionic detergents are used, the detergent concentration and the solubilization time must be first optimized. Usually the detergent concentration range from 0.5%-5% (v/v).
      CAUTION: Digitonin is toxic, wear protective clothes and gloves
3. Remove the insolubilized material by centrifugation at 18,000 x g for 20 min, at +4 ˚C.
4. Transfer the supernatant to a new 1.5 mL tube and add 1/10 (v/v) of CBB buffer to the sample.
   NOTE: When the overall composition of the thylakoid membrane protein complexes is examined, determining the yield and the represented thylakoid domain of solubilized fraction is recommended. To determine the yield of the solubilized material, take 5 µL of the supernatant to a new tube (before adding CBB) and measure the Chl content and Chl a/b ratio⁵.

3. BN-PAGE^1,2,3

1. Set up the gel to a vertical electrophoresis system (e.g., Hoefer SE 250). Fill the upper buffer chamber with blue cathode buffer (see Table 1) and pour anode buffer to the lower buffer chamber.
2. Load thylakoid sample (e.g., 5 µg of chlorophyll) into the wells.
3. Start the electrophoresis and gradually increase the voltage: 75 V for 30 min, 100 V for 30 min, 125 V for 30 min, 150 V for 1 h and 175 V until the complexes have been separated completely. Run the gel at +4˚C, either using a cold room or adjusting the gel temperature with a cooling system.
   NOTE: Change the blue cathode buffer to a clear cathode buffer when the sample front has migrated about one third of the gel.
4. After the electrophoretic run, scan the gel with a photoscanner for image archiving.

4. 2D-SDS-PAGE

1. Assemble the vertical electrophoresis system (gel size 16 cm x 20 cm). Use 1 mm spacers.
2. Prepare standard SDS gel (12% acrylamide, 6 M Urea, see recipe in Table 2) with a 2D-comb (single large well for the strip and one standard well for molecular weight marker).
3. Cut the lane from BN-gel and place it in a (5 mL) tube. Add 2 mL of Laemmli buffer (containing 5% β-mercaptoethanol) and incubate the strip for 45 min with gentle shaking at RT.
4. Place the lane, with a help of e.g., a spacer, on top of the gel avoiding air bubbles.
5. Pipet 5 µL of molecular weight marker on a narrow piece of filter paper and place the paper to the standard well.
6. To seal the BN-gel strip and the marker paper, pour 0.5% agarose (in running buffer) on top of the gel strip and allow to solidify.
7. Perform electrophoresis according to standard protocols. After the electrophoretic run, visualize the proteins with e.g., Sypro Ruby stain or silver staining according to⁶.

Wyniki

A representative 2D-BN/SDS-PAGE system in Figure 1 demonstrates the separation of digitonin and β-DM-solubilized thylakoid protein complexes and their detailed protein subunit composition. The protein complex pattern of digitonin solubilized thylakoids (horizontal gel on the top on the top of Figure 1A) contains the PSII-LHCII-PSI megacomplex, two large PSII-LHCII supercomplexes (sc), PSI-LHCII supercomplex, PSI monomer (m),...

Dyskusje

The photosynthetic energy conversion machinery is composed of large multisubunit protein complexes, which are embedded in the thylakoid membrane. This protocol describes a basic method for analysis of the plant thylakoid protein complexes from Arabidopsis thaliana with BN-PAGE combined with 2D-SDS-PAGE. The protocol is also suitable for the analysis of thylakoid protein complexes from tobacco and spinach thylakoids, but might require small adjustments.

For the solubilization of membra...

Materiały

Name	Company	Catalog Number	Comments
6-aminocaproic acid (ACA)	Sigma-Aldrich	A2504
BisTris	Sigma-Aldrich	B4429
Sucrose	Sigma-Aldrich	S0389
Acrylamide (AA)	Sigma-Aldrich	A9099	Caution: Neurotoxic!
n-dodecyl-β-D-maltoside	Sigma-Aldrich	D4641
Tricine	Sigma-Aldrich	T0377
Tris	Sigma-Aldrich	T1503
SDS	VWR	442444H
Urea	VWR	28877.292
Glycerol	J.T. Baker	7044
Sodium Fluoride (NaF)	J.T. Baker	3688
EDTA disodium salt	J.T. Baker	1073
Digitonin	Calbiochem	300410	Caution:Toxic!
Pefabloc SC	Roche	11585916001
Serva Coomassie Blue G	Serva	35050
β-mercaptoethanol	Bio-Rad	1610710
APS (Ammonium persulfate)	Bio-Rad	161-0700
TEMED (Tetramethylethylenediamine)	Bio-Rad	1610801
(N,N'-Methylene)-Bis-Acrylamide	Omnipur	2610
Glycine	Fisher	G0800
Prestained Protein Marker, Broad Range (7-175 kDa)	New England Biolabs	P7708
Falcon, Conical Centrifuge Tubes 15 ml	Corning	352093
Dual gel caster with 10 x 8 cm plates	Hoefer	SE215
Gradient maker SG5	Hoefer
0.75 mm T-spacers	Hoefer	SE2119T-2-.75
Sample gel comb, 0.75 mm	Hoefer	SE211A-10-.75
Mighty Small SE250 vertical electrophoresis system	Hoefer	SE250
IPC-pump	Ismatec
Power supply, PowerPac HV	Bio-Rad	164-5097
Centrifuge	Eppendorf	5424R
Rocker-Shaker	Biosan	BS-010130-AAI
PROTEAN II xi Cell	Bio-Rad	1651813