Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
Please note that all translations are automatically generated. Click here for the English version.
8.4K Views
•
08:31 min
•
October 3rd, 2018
DOI :
October 3rd, 2018
•Przeglądaj więcej filmów
Rozdziały w tym wideo
0:04
Title
0:33
Anaerobic DesB Purification
2:29
Anaerobic Protein Concentration, Buffer Exchange, and DesB Desalting
4:10
Oxygen Electrode Preparation
5:41
Oxygen Electrode Kinetic Assay
6:48
Results: Representative DesB Dioxygenase Induction, Activity, and Inhibition
7:50
Conclusion
Powiązane Filmy
12:08
Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry
15.1K Views
09:54
Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana
15.3K Views
08:57
Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
10.0K Views
09:00
Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
10.0K Views
08:02
Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory
17.2K Views
10:14
Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
6.3K Views
11:32
Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates
2.6K Views
08:19
Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry
2.3K Views
05:59
Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
2.5K Views
08:03
Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays
478 Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone