Anaerobic Protein Concentration, Buffer Exchange, and DesB Desalting
4:10
Oxygen Electrode Preparation
5:41
Oxygen Electrode Kinetic Assay
6:48
Results: Representative DesB Dioxygenase Induction, Activity, and Inhibition
7:50
Conclusion
필기록
This method is designed to answer key questions in the field inorganic biochemistry, about how to maintain enzyme activity in proteins that contain oxygen-sensitive metals. The main advantage of this technique is that it helps you visualize ways i
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
Here we present a protocol for anaerobic protein purification, anaerobic protein concentration, and subsequent kinetic characterization using an oxygen electrode system. The method is illustrated using the enzyme DesB, a dioxygenase enzyme which is more stable and active when purified and stored in an anaerobic environment.