The ECLIA is a simple yet efficient method to quantify cellular levels of MeCP2. The ECLIA is fast, more sensitive, and easier to handle compared to other quantification methods such as the Western blot and the ELISA. To prepare the washing soluti
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The electrochemiluminescence immunoassay (ECLIA) is a novel approach for quantitative detection of endogenous and exogenously applied MeCP2 protein variants, which produces highly quantitative, accurate and reproducible measurements with low intra- and inter-assay error over a wide working range. Here, the protocol for the MeCP2-ECLIA in a 96-well format is described.