An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
27.0K Views
•
07:44 min
•
May 22nd, 2020
DOI :
May 22nd, 2020
•Hannes Steinkellner1, Alexander V. Beribisky1, Philip Mausberg1, John Christodoulou2, Barbara Scheiber-Mojdehkar3, Anna Huber1, Victoria Sarne1, Franco Laccone1
1Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna (MUV), 2Murdoch Children's Research Institute and Department of Paediatrics, University of Melbourne, Discipline of Child & Adolescent Health, Sydney Medical School, 3Department of Medical Chemistry and Pathobiochemistry, Medical University of Vienna
Chapters in this video
0:04
Introduction
0:35
Preparing Solutions and Plates (Day 1)
1:50
Blocking and Sample Addition (Day 2)
3:42
Antibody Addition
5:17
Reading the Plate
6:10
Results: Accuracy and Consistency of MeCP2-ECLIA
7:20
Conclusion
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