Methods Article
GM-Free Generation of Blood-Derived Neuronal Cells
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08:11 min
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February 13th, 2021
DOI :
February 13th, 2021
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Rozdziały w tym wideo
0:04
Introduction
0:47
Isolation of Human Peripheral Blood Mononuclear Cells (PBMNCs
1:25
Activation of Human GPI-Anchored Glycoprotein by Antibody Crosslinking on the Surface of PBMNCs
2:21
Sorting of Newly Generated Dedifferentiated Cells
3:33
Preparing Cell Culture Dishes for Neuronal Differentiation of Newly Generated Stem Cells
4:10
Culturing of Neuronal Differentiated Blood Cells
4:44
Immunofluorescence Microscopy Analysis of Blood-Derived Neural Cells
6:08
Results: Morphology, Re-differentiation and Immunophenotyping of Generated Neuronal Cells
7:41
Conclusion
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