Isolation of Human Peripheral Blood Mononuclear Cells (PBMNCs
1:25
Activation of Human GPI-Anchored Glycoprotein by Antibody Crosslinking on the Surface of PBMNCs
2:21
Sorting of Newly Generated Dedifferentiated Cells
3:33
Preparing Cell Culture Dishes for Neuronal Differentiation of Newly Generated Stem Cells
4:10
Culturing of Neuronal Differentiated Blood Cells
4:44
Immunofluorescence Microscopy Analysis of Blood-Derived Neural Cells
6:08
Results: Morphology, Re-differentiation and Immunophenotyping of Generated Neuronal Cells
7:41
Conclusion
Transcript
The significance of the protocol relies on membrane to nucleus signaling and does not directly affect the genome, like it is the case with induced pluripotent stem cells. The non-teratogenic nature of blood-derived pluripotent stem cells is the ma
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We present a genetically modified-free (GM-free) method to obtain cells with a neuronal phenotype from reprogrammed peripheral blood cells. Activation of a signaling pathway linked to novel human GPI-linked protein reveals an efficient GM-free method for obtaining human pluripotent stem cells.
