Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments

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1.6K Views

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11:24 min

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February 3rd, 2023

DOI :

10.3791/64650-v

February 3rd, 2023

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Francisca M. Acosta¹, Maria A. Gonzalez Porras²^,³, Katerina Stojkova², Settimio Pacelli², Christopher R. Rathbone²^,³, Eric M. Brey²^,³

¹Department of Biochemistry and Structural Biology, University of Texas Health Science Center, ²Department of Biomedical Engineering and Chemical Engineering, University of Texas at San Antonio, ³Institute of Regenerative Medicine, University of Texas at San Antonio

Podsumowanie

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Three Dimensional Culture

Vascularized Adipose Tissue

Thermogenic Fat

Microvascular Fragments

Inguinal Fat Isolation

Epididymal Fat Harvesting

Subcutaneous Fat Isolation

BSA In PBS

Biological Imitations

Tissue Engineering

Rozdziały w tym wideo

0:04

Introduction

0:48

Isolation of Inguinal, Epididymal, and Posterior Subcutaneous Fat from Rat

3:00

Isolation of Microvascular Fragment (MVF)

7:40

Casting of the MVF Fibrin Gel

8:37

Results: Assessment of the Adipose Tissue Formed from the MVFs

10:46

Conclusion

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