Isolation of Inguinal, Epididymal, and Posterior Subcutaneous Fat from Rat
3:00
Isolation of Microvascular Fragment (MVF)
7:40
Casting of the MVF Fibrin Gel
8:37
Results: Assessment of the Adipose Tissue Formed from the MVFs
10:46
Conclusion
Transcrição
This protocol describes the generation of three-dimensional vascularized and functional thermogenic fat from microvascular fragments, or MVFs. Current strategies for engineering thermogenic adipose tissue are complex and don't fully depict its mul
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Here, we present a detailed protocol outlining the use of microvascular fragments isolated from rodent or human fat tissue as a straightforward approach to engineer functional, vascularized beige adipose tissue.