Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples
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05:26 min
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February 3rd, 2023
DOI :
February 3rd, 2023
•Roberto Plebani1,2, Alessandra D'Alessandro2, Paola Lanuti2,3, Pasquale Simeone2,3, Massimiliano Cinalli1, Ilaria Righi4, Alessandro Palleschi4,5, Matteo Mucci1,2, Marco Marchisio2,3, Francesco Cappabianca1, Marina Camera6,7, Felice Mucilli1, Mario Romano1,2
1Department of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, 2Center on Advanced Studies and Technology (CAST), “G. d’Annunzio” University of Chieti-Pescara, 3Department of Medicine and Aging Sciences, University “G. d’Annunzio” of Chieti-Pescara, 4U.O. di Chirurgia Toracica e dei Trapianti di Polmone, Fondazione IRCCS Ca’ Granda - Ospedale Maggiore Policlinico di Milano, 5Dipartimento di Fisiopatologia Medico-Chirurgica e dei Trapianti, Università degli Studi di Milano, 6Department of Pharmaceutical Sciences, Università degli Studi di Milano, 7Unit of Cell and Molecular Biology in Cardiovascular Diseases, Centro Cardiologico Monzino IRCCS
Rozdziały w tym wideo
0:04
Introduction
0:45
Sample Preparation and Enzymatic Digestion
1:47
Recovery of the Digested Cells and Removal of Non-Endothelial Cells (EC) by Filtration
2:32
Sedimentation of Cell Clusters and Cell Seeding
3:16
Results: Purification and Characterization of Freshly Isolated HLMVECs
4:30
Conclusion
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