June 23rd, 2010
•We describe a method to separate organelles by density in living Drosophila embryos. Embryos are embedded in agar and centrifuged. This technique yields reproducible separation of major organelles along the anterior-posterior embryo axis. This method facilitates colocalization experiments and yields organelle fractions for biochemical analysis and transplantation experiments.
Vídeos Relacionados
Preparation of Neuronal Cultures from Midgastrula Stage Drosophila Embryos
The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol
Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila
Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube
Upright Imaging of Drosophila Embryos
Primary Cell Cultures from Drosophila Gastrula Embryos
In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution
RNAi Interference by dsRNA Injection into Drosophila Embryos
Imaging Cell Shape Change in Living Drosophila Embryos
Isolation and Purification of Kinesin from Drosophila Embryos
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados