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JoVE Journal

Neuroscience

This content is Open Access.

Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish

16.5K Views

12:43 min

October 17th, 2012

October 17th, 2012

16,481 Views

0:05

Title

1:52

Pre-experimental Preparations

2:47

Isolation of Auditory and Vestibular Labyrinth

5:28

Hair Cell Isolation

7:18

Patch Clamping Zebrafish Hair Cells

10:12

Results: Isolation of and Electrophysiological Recordings from Zebrafish Hair Cells

12:04

Conclusion

The purpose of this video is to demonstrate procedures for obtaining healthy, intact hair cells from the inner ear organs of adult zebrafish and then using them for patch clamp studies aimed at characterizing the biophysical properties of their voltage-gated channels.

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