October 3rd, 2018
•The protocols described allow the construction, characterization and selection (against the target of choice) of a "domainome" library made from any DNA source. This is achieved by a research pipeline that combines different technologies: phage display, a folding reporter and next generation sequencing with a web tool for data analysis.
Tags
Vídeos Relacionados
Primer-Free Aptamer Selection Using A Random DNA Library
Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects
Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity
A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Targeted DNA Methylation Analysis by Next-generation Sequencing
Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
3' End Sequencing Library Preparation with A-seq2
Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados