Preparation and Characterization of si-NPs (siRNA Nanoparticles)
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Physicochemical Characterization of si-NPs
5:04
Determining in vitro Bioactivity of si-NPs
6:35
Results: Comparison between si-NP 1 and si-NP 2 and siRNA Complexation
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Conclusion
Transcrição
The current protocol is significant because it allows researchers to produce siRNA loaded nanoparticles, and to adequately characterize these nanoparticles to ensure their suitability for administration to animals prior to subsequent studies. The
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Methods to prepare and characterize the physicochemical properties and bioactivity of neutrally-charged, pH-responsive siRNA nanoparticles are presented. Criteria for successful siRNA nanomedicines such as size, morphology, surface charge, siRNA loading, and gene silencing are discussed.