Oligonucleotide Library Design and Preparation and Bacteria Culture
2:07
Nuclease Activity Assay Setup
3:05
Measurement Setup
4:32
Data Analysis
5:12
Results: Representative Kinetic Screening
6:23
Conclusion
TRANSCRIÇÃO
This protocol provides a powerful alternative for screening nuclease activity as biomarker of disease, with an easy-to-implement methodology even for researchers who are not as specialized in nucleic acid probes. The main advantage of this techniq
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Altered nuclease activity has been associated with different human conditions, underlying its potential as a biomarker. The modular and easy to implement screening methodology presented in this paper allows the selection of specific nucleic acid probes for harnessing nuclease activity as a biomarker of disease.