Collection, Isolation and Quantification of MEVs (Mycobacterial Extracellular Vesicles)
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Results: MEV Purification and Quantification
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Conclusion
Transcrição
This method permits culturing Mycobacterium tuberculosis under strict low iron conditions and purifying membrane vesicles from the low iron culture filtrate. Because iron limitation is known to stimulate membrane vesicle production, this protocol
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Mycobacterium tuberculosis shows increased production and release of extracellular vesicles in response to low iron conditions. This work details a protocol for generating low iron conditions and methods for the purification and characterization of mycobacterial extracellular vesicles released in response to iron deficiency.