Culturing Conditions and Extracellular Vesicles Isolation
5:15
Extracellular Vesicles (EVs) Isolation with Size Exclusion Chromatography (SEC)
7:15
EV Preparation Quality Control and Storage
7:58
Results: Analysis of EVs Fraction Collected from Escherichia coli MP1
9:24
Conclusion
TRANSCRIÇÃO
This protocol is important because it provides a way to isolate extracellular vesicles from a variety of bacteria in a highly reproducible manner. The great thing about this technique is it can be used to isolate EVs from quite large environments
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Bacteria secrete nanometer-sized extracellular vesicles (EVs) carrying bioactive biological molecules. EV research focuses on understanding their biogenesis, role in microbe-microbe and host-microbe interactions and disease, as well as their potential therapeutic applications. A workflow for scalable isolation of EVs from various bacteria is presented to facilitate standardization of EV research.