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JoVE Journal

Immunology and Infection

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Assessment of Acute Wound Healing using the Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation and Excisional Tail Skin Wound Models.

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09:06 min

March 25th, 2020

March 25th, 2020

7,874 Views

0:05

Introduction

0:53

Subcutaneous Polyvinyl Alcohol (PVA) Sponge Implantation

3:09

PVA Sponge Fluid Isolation

3:58

PVA Sponge Cell Isolation

5:33

Tail Skin Excision

6:57

Results: Representative Assessment of Acute Wound Healing

8:40

Conclusion

Transcrição

These models can be implemented to assess various aspects of the wound healing response, including cellular and cytokine kinetics and wound closure. The PVA sponge model allows the recovery of millions of wound leukocytes for phenotypic and functi

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Here, two murine wound healing models are described, one designed to assess cellular and cytokine wound healing responses and the other to quantify the rate of wound closure. These methods can be used with complex disease models such as diabetes to determine mechanisms of various aspects of poor wound healing.

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