Biotinylation of the NKG2D Fusion Protein and Thawing of Primary AML Cells
2:00
Staining of Primary AML Cells with the Biotinylated NKG2D Fusion Protein
3:24
Results: Gating Strategy and Staining Protocol Comparison
4:49
Conclusion
TRANSCRIÇÃO
This protocol shows two distinct ways of detecting NKG2DLs on the surface of AML cells. The technique provides a fast, user-friendly staining method to detect all known and possibly unknown NKG2DLs. This method allows for separation of leukemic st
Sign in or start your free trial to access this content
We present two different staining protocols for NKG2D ligand (NKG2DL) detection in human primary acute myeloid leukemia (AML) samples. The first approach is based on a fusion protein, able to recognize all known and potentially yet unknown ligands, while the second protocol relies on the addition of multiple anti-NKG2DL antibodies.