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Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia

4.0K Views

05:24 min

February 21st, 2021

DOI :

10.3791/61803-v

February 21st, 2021


Transcript

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NKG2D Ligands

Chapters in this video

0:04

Introduction

0:43

Biotinylation of the NKG2D Fusion Protein and Thawing of Primary AML Cells

2:00

Staining of Primary AML Cells with the Biotinylated NKG2D Fusion Protein

3:24

Results: Gating Strategy and Staining Protocol Comparison

4:49

Conclusion

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