July 6th, 2022
•Here, we present a protocol to significantly reduce the mitochondrial DNA copy numbers in a bovine oocyte (P < 0.0001). This method utilizes centrifugation and bisection to substantially reduce oocyte mitochondria and may allow for an increased chance of development in the reconstructed interspecies somatic cell nuclear transfer embryos.
Vídeos Relacionados
Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays
Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration
Functional Cloning Using a Xenopus Oocyte Expression System
Technique to Target Microinjection to the Developing Xenopus Kidney
Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish
A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro
Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo
Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados