Isolation of the Tibialis Anterior (TA) and the Extensor Digitorum Longus (EDL) Muscles
3:17
Isolation of the Gastrocnemius (GA) and Soleus Muscles
4:27
Isolation of the Triceps Muscles
5:07
Isolation of the Masseter Muscles
6:08
Isolation of the Diaphragm Muscle
7:20
Muscle Digestion to a Single‐Cell Suspension
8:40
Results: Purification, Purity Assessment, and Quantitative Measurement of Quiescent Stem Cell Populations from Individual Skeletal Muscles
10:47
Conclusion
Transcrição
This protocol is significant because it enables the study of stem cell function across different skeletal muscles, deepening our understanding of how they contribute to homeostasis, regeneration, and disease progression. Using this protocol, we ca
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This protocol describes the isolation of muscle stem cells and fibro-adipogenic progenitors from individual skeletal muscles in mice. The protocol involves single muscle dissection, stem cell isolation by fluorescence activated cell sorting, purity assessment by immunofluorescence staining, and quantitative measurement of S-phase entry by 5-ethynyl-2´-deoxyuridine incorporation assay.