Isolation of Quiescent Stem Cell Populations from Individual Skeletal Muscles

2.9K Views

11:35 min

December 9th, 2022

DOI :

10.3791/64557-v

December 9th, 2022


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Quiescent Stem Cells

Chapters in this video

0:04

Introduction

0:35

Isolation of the Gracilis Muscles

1:26

Isolation of the Tibialis Anterior (TA) and the Extensor Digitorum Longus (EDL) Muscles

3:17

Isolation of the Gastrocnemius (GA) and Soleus Muscles

4:27

Isolation of the Triceps Muscles

5:07

Isolation of the Masseter Muscles

6:08

Isolation of the Diaphragm Muscle

7:20

Muscle Digestion to a Single‐Cell Suspension

8:40

Results: Purification, Purity Assessment, and Quantitative Measurement of Quiescent Stem Cell Populations from Individual Skeletal Muscles

10:47

Conclusion

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