This work was funded by a DBT/Wellcome Trust (India Alliance) grant (IA/S/19/2/504632) to S.S. P.N. is a Research Fellow supported by a DBT/Wellcome Trust (India Alliance) grant (IA/S/19/2/504632). A.M. is supported by the Council of Scientific and Industrial Research (CSIR), Government of India, as a Senior Research Fellow (09/087(0873)/2017-EMR-I). The authors thank Paike Jayadeva Bhat for discussions.

NOTE: The protocol broadly involves the following steps: (1) patching the haploids in the mating efficiency grids on a YPD plate, (2) mixing the haploids in equal numbers after 24 h incubation and giving the mixed haploids a few hours to mate (7 h in this study), (3) plating the mixed cells on YPD for isolating single colonies after 7 h at 30 &#176;C, and finally, (4) determining the number of diploids formed using the auxotrophic markers. These steps are discussed in detail below (also see Figure 2<...

<ol>
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The quantification of the mating efficiency in S. cerevisiae is essential for carrying out studies related to the genes involved in mating pathways or studying the influence of the external environment on mating behavior. In the past two decades, S. cerevisiae has also become a popular model to address questions related to speciation14,36,37,38. The presence of two mating type...

Saccharomyces cerevisiae, commonly called budding yeast, is a unicellular eukaryote. It has two mating types, a and &#945;, and exhibits both asexual and sexual reproductive cycles. The a and &#945; mating types are haploids and can divide mitotically in the absence of the other mating type in the surrounding environment, which represents the asexual cycle of yeast. When the two mating types are in close proximity, they stop dividing mitotically and fuse to form a diploid cell. The diploid yeast can either divide mitotically when nutrients are present or undergo meiosis under the conditions of nitrogen starvation in the presence of a poor carbon source that is non-fermentable, such as acetate1. This results in the formation of spores, which remain dormant until there are favorable growth conditions. The life cycle is completed when these spores germinate and the two haploid types are released back to the haploid pool2,3 (Figure 1).

The mating of yeast cells includes several steps, such as agglutination, the formation of a mating projection or &#8220;shmoo&#8221;, followed by cell and nuclear fusion4,5. The two mating types a and &#945; produce a-factor and &#945;-factor, respectively, to initiate mating. These factors are polypeptide pheromones that bind to the receptors (Ste2 and Ste3) present on the cell surface of the opposite mating type5. The binding of the pheromones to the receptors initiates the pheromone response pathway, the mitogen-activated protein kinase (MAPK) signal transduction pathway6,7,8. This results in the arrest of the cell cycle in the G1 phase, leading to a metabolically active stationary phase9. The cells then stop dividing mitotically, and the proteins required for mating are synthesized. As the haploid cells cannot move toward each other, a mating projection or &#8220;shmoo&#8221; is directed toward the mating partner. When the cells come into contact, the cell wall is degraded, and the cytoplasmic contents fuse, resulting in mating to form a diploid cell10,11. The mating efficiency between haploids has been used as a measure of speciation in laboratory-evolved strains, as well as between extant species12.

Being a simple eukaryotic organism, yeast is the model of choice for a large number of research questions associated with complex eukaryotic organisms. One such question is associated with speciation and the evolution of reproductive barriers13,14. For sexually reproducing organisms, a species is defined by the biological species concept (BSC) proposed by Ernst Mayr15. According to this concept, two individuals of a population are said to belong to two different species if they cannot interbreed and are reproductively isolated. Breakdown of the sexual reproductive cycle (which involves the fusion of gametes to form a zygote, the development of the zygote into a progeny, and the attainment of sexual maturity in the progeny) leads to reproductive isolation. As shown in Figure 1, the life cycle of S. cerevisiae is comparable to the sexual reproductive cycle: a) the fusion of the two mating types a and &#945; is similar to the fusion of gametes in sexually reproducing organisms; b) the ability of the diploid to undergo mitotic division is equivalent to the zygote developing into progeny; and c) the diploid undergoing sporulation is comparable to the process of gametogenesis14.

Pre-zygotic isolation occurs when assortative mating is observed. Given an equal opportunity to mate with two genetically different a types, an &#945; type preferentially mates with one over the other or vice versa14. In the case of evolution experiments in which haploids have been evolved in different environments, the presence of a pre-mating barrier can be determined by performing a mating assay. A decrease in the mating efficiency when compared to the ancestor indicates the evolution of a pre-mating barrier. Post-zygotic isolation could arise due to the inability of the diploid to undergo effective mitotic division and/or sporulation to form haploid spores14. These can be quantified by measuring the growth rate of the diploids and calculating the sporulation efficiency, respectively. Hence, to study the evolution of reproductive barriers, robust methods for quantifying (a) the mating efficiency, (b) the mitotic growth of the diploid, and (c) the sporulation efficiency of the diploid are required. In this work, a robust method to quantify the mating efficiency of yeast strains is reported.

In laboratory experiments, one of the ways in which the occurrence of mating can be detected is by using auxotrophic markers that complement the nutritional requirements. When the two mating types are auxotrophic for two different amino acids, only the diploid cell formed by the fusion of the two mating types can grow on a medium deficient in both the amino acids. Thus, auxotrophic markers are useful for detecting mating both qualitatively and quantitatively. A qualitative test will suffice to identify the mating type of a strain after meiosis16. Quantitative tests are essential when one is interested in identifying a reduction in mating while studying the genes involved in the mating pathway17,18. In addition, with yeast being increasingly used in speciation studies, a convenient and reproducible mating assay is necessary, as the quantification of mating efficiency is a measure of the pre-zygotic barrier.

The mating efficiency between the two yeast mating types has been quantified previously16,19,20. Most of the previously used methods are similar in their design with a few variations16,21,22,23,24,25. Some of them use early log phase cultures, while a few others use mid-log phase cultures of haploid strains. There are variations in the ratios in which the two mating types are mixed. Almost all the protocols use a nitrocellulose membrane. Suspensions of both the mating types taken from previously grown cultures are mixed and filtered onto a nitrocellulose membrane placed on a YPD plate. In one of the variations of the protocol, the haploid suspension is directly patched on a YPD plate21. In experiments dealing with the genes involved in the pheromone production of the two mating types, the pheromones are externally added while making the suspensions of the two mating types24.

Following incubation for a few hours (typically around 5 h) after mixing the haploids, the cells are washed off from the membrane, diluted, and plated on selective media. In one of the earlier methods reported in 1973, the efficiency of zygote formation or mating was calculated by counting the number of budded cells, unbudded cells, and mating pairs under a microscope using a hemocytometer26. However, most methods reported later use auxotrophic markers to distinguish haploids and diploids. Mating efficiency is calculated as the percentage of diploid cells relative to the number of diploid and haploid cells in the cellular pool16,21,23.

However, despite a number of reports using yeast as a model organism to study speciation, there is no standardized protocol reported in the literature until now for calculating the efficiency of mating. Cells in the log phase may not be ideal for the quantification of mating efficiency. During mating, the cell cycle of the two haploids is arrested, and hence, the cells during mating are not dividing9. As the cell cycle is also known to be similarly arrested in cells in the stationary phase27, using such cells can make the protocol more reproducible. Stationary phase cells can be mixed and laid out on YPD plates (i.e., a nutritionally rich environment) for mating. The conventional procedures also require a nitrocellulose membrane and washing off the cells, making the process cumbersome and liable to handling errors. In addition, the protocols used to date quantify the mating efficiency in terms of one haploid. However, when measuring reproductive isolation, mating efficiency is quantified for a particular combination of haploids rather than a single haploid.

To address these issues, here, we report a robust method for the quantification of mating efficiency in yeast that is highly reproducible and easy to use. Moreover, this method and the yeast strains employed here can also be used in studies examining the effect of gene flow on the evolution of mating barriers.

Two different strains of S. cerevisiae were used in this study. One of the strains is derived from the SK1 background; this was modified in our laboratory by adding the auxotrophic markers near the MAT locus. The resulting genotypes of the haploids are provided in Table 128,29,30. In the SK1 strain, the a haploid had the TRP1 gene inserted near the MAT locus, and the &#945; haploid had the LEU2 gene inserted near the MAT locus. In the ScAM strain, the TRP1 and URA3 genes were inserted in the a and &#945; haploids, respectively. The location of insertion was in the ARS region of chromosome III (Chr III: 197378..197609). For the protocol reported here, auxotrophic markers anywhere on the genome would suffice. However, having the auxotrophic markers near the MAT locus means that these strains can also be used for studies examining the effect of gene flow on speciation31,32. The markers were added close to the MAT locus to prevent the reshuffling of the markers due to recombination. Hence, this protocol can be used to quantify mating efficiency in studies involving speciation and also to identify the alteration of mating efficiency when studying the proteins involved in the mating pathway.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Adenine</td>
			<td>Sigma Life Science</td>
			<td>A8626</td>
			<td></td>
		</tr>
		<tr>
			<td>Agar Powder regular grade for bacteriology</td>
			<td>SRL</td>
			<td>19661 (0140186)</td>
			<td></td>
		</tr>
		<tr>
			<td>Ammonium Sulphate, Hi-AR</td>
			<td>HiMedia</td>
			<td>GRM1273</td>
			<td></td>
		</tr>
		<tr>
			<td>D-(+)-glucose</td>
			<td>Sigma Life Science</td>
			<td>G8270</td>
			<td></td>
		</tr>
		<tr>
			<td>Glass Petri plates</td>
			<td>HiMedia</td>
			<td>PW008</td>
			<td>&#160;90 mm x 15 mm dimension</td>
		</tr>
		<tr>
			<td>L-Arginine</td>
			<td>Sigma Life Science</td>
			<td>A8094</td>
			<td></td>
		</tr>
		<tr>
			<td>L-Aspartic acid</td>
			<td>Sigma Life Science</td>
			<td>A7219</td>
			<td></td>
		</tr>
		<tr>
			<td>L-Histidine monochloride monohydrate</td>
			<td>Sigma Life Science</td>
			<td>H5659</td>
			<td></td>
		</tr>
		<tr>
			<td>L-Isoleucine</td>
			<td>Sigma Aldrich</td>
			<td>I2752</td>
			<td></td>
		</tr>
		<tr>
			<td>L-Leucine</td>
			<td>Sigma Life Science</td>
			<td>L8912</td>
			<td></td>
		</tr>
		<tr>
			<td>L-Lysine</td>
			<td>Aldrich</td>
			<td>62840</td>
			<td></td>
		</tr>
		<tr>
			<td>L-Methionine</td>
			<td>Sigma Life Science</td>
			<td>M5308</td>
			<td></td>
		</tr>
		<tr>
			<td>L-Phenylalanine</td>
			<td>Sigma Life Science</td>
			<td>P5482</td>
			<td></td>
		</tr>
		<tr>
			<td>L-Threonine</td>
			<td>Sigma Aldrich</td>
			<td>T8625</td>
			<td></td>
		</tr>
		<tr>
			<td>L-Tyrosine</td>
			<td>Sigma Life Science</td>
			<td>T8566</td>
			<td></td>
		</tr>
		<tr>
			<td>L-Valine</td>
			<td>Sigma Life Science</td>
			<td>V0513</td>
			<td></td>
		</tr>
		<tr>
			<td>Mating efficiency grid</td>
			<td></td>
			<td></td>
			<td>1 cm x 1.5 cm rectangular grid drawn on the Petri plate</td>
		</tr>
		<tr>
			<td>Microcentrifuge tubes</td>
			<td>Tarsons</td>
			<td>500010</td>
			<td></td>
		</tr>
		<tr>
			<td>Peptone</td>
			<td>HiMedia</td>
			<td>RM001</td>
			<td></td>
		</tr>
		<tr>
			<td>Uracil</td>
			<td>Sigma Life Science</td>
			<td>U0750</td>
			<td></td>
		</tr>
		<tr>
			<td>Yeast Extract Powder</td>
			<td>HiMedia</td>
			<td>RM027</td>
			<td></td>
		</tr>
		<tr>
			<td>Yeast Nitrogen Base w/o Amino acids and Ammonium Sulphate</td>
			<td>BD Difco</td>
			<td>233520</td>
			<td></td>
		</tr>
	</tbody>
</table>

determination of the mating efficiency of haploids in saccharomyces cerevisiae

Saccharomyces cerevisiae is a widely used model organism in genetics, evolution, and molecular biology. In recent years, it has also become a popular model organism to study problems related to speciation. The life cycle of yeast involves both asexual and sexual reproductive phases. The ease of performing evolution experiments and the short generation time of the organism allow for the study of the evolution of reproductive barriers. The efficiency with which the two mating types (a and &#945;) mate to form the a/&#945; diploid is referred to as the mating efficiency. Any decrease in the mating efficiency between haploids indicates a pre-zygotic barrier. Thus, to quantify the extent of reproductive isolation between two haploids, a robust method to quantify the mating efficiency is required. To this end, a simple and highly reproducible protocol is presented here. The protocol involves four main steps, which include patching the haploids on a YPD plate, mixing the haploids in equal numbers, diluting and plating for single colonies, and finally, calculating the efficiency based on the number of colonies on a drop-out plate. Auxotrophic markers are employed to clearly make the distinction between haploids and diploids.

Quantification of the mating efficiency of the two mating types 
The protocol described here was used to quantify the mating efficiency between two yeast strains-between SK1AMa and SK1AM&#945; and between ScAMa and ScAM&#945; (Figure 3A). In these experiments, the mating between the two haploids was repeated at least 12 times. In each of the repeats of the experiment, at least 100 colonies were streaked...

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Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae

In this work, a robust method for the quantification of mating efficiency in the yeast Saccharomyces cerevisiae is described. This method is particularly useful for the quantification of pre-zygotic barriers in speciation studies.

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae

Saccharomyces cerevisiae is a widely used model organism in genetics, evolution, and molecular biology. In recent years, it has also become a popular ...

This protocol is a simple method to quantify the efficiency with which different strains and species of yeast mate with each other. The proposed method is simple, robust, and efficient. The method can be extended to any strain of yeast.The method described in this study can be used to understand how speciation occurs in yeast. Moreover, the strains that we describe can be particularly useful to design experiments with gene flow. The method's strength is that it's really simple.Some slight adjustments like the time of incubation might be needed depending on the particular strain used. Begin reviving the haploids A and Alpha from frozen stocks by streaking the yeast inoculum on a yeast extract peptone dextrose, or YPD agar plate. Allow them to grow for 48 hours at 30 degrees Celsius to obtain single colonies.After incubation, inoculate single colonies from the YPD plate and five milliliters of YPD medium, and incubate at 30 degrees Celsius for 48 hours with 250 RPM shaking. The cells are in the stationary phase of growth after this incubation period. On a fresh YPD plate, draw mating efficiency grid as a 1 by 1.5 centimeter rectangle divided into three boxes, each with a dimension of 1 by 0.5 centimeter.Inoculate five microliters of the YPD culture of the two mating types on the leftmost and rightmost rectangles. This volume corresponds to roughly 5 times 10 to the fifth haploid cells in each section. Incubate the plates for 24 hours at 30 degrees Celsius.To mix an equal number of yeast cells, remove about 1/3 of the yeast growth developed in the outer boxes using a sterile toothpick and resuspend the removed cells in 20 microliters of water in a sterile 1.5 milliliter vial. Follow this for each haploid. Then, dilute five microliters of this suspension in two milliliters of water and measure the optical density using a spectrophotometer at 600 nanometers.Based on the optical density value and the number of cells per milliliter at one optical density, add the required volumes of both haploids in a fresh and sterile 1.5-milliliter vial. Mix it well using a pipette. The final volume of this suspension is generally around six to eight microliters.Next, inoculate this suspension in the center grid. Incubate the plate at 30 degrees Celsius for seven hours, allowing the haploids to mate. After seven hours of incubation, scrape the cells from the center rectangle using a toothpick or a pipette tip and dilute in two milliliters of sterile water.Carry out further dilutions, and then spread the cell suspension on YPD agar to obtain single colonies. After plating, incubate the YPD plates at 30 degree Celsius for 36 to 48 hours to develop single colonies. Ensure that a few hundred individual colonies are obtained from each mating experiment for screening to ensure the statistical significance of the data.To identify the diploid colonies on the plate, transfer the single colonies by streaking them individually onto a double drop-out plate, lacking the amino acids that the strains are auxotrophic for. Incubate the plates at 30 degrees Celsius for 48 hours and calculate the mating efficiency, nu. The robustness of the protocol enabled easy differentiation of the mating efficiency between the two strains, SK1AM and ScAM.While the SK1 strains mated with a very high efficiency, the ScAM strains mated with a relatively lower efficiency. The mating efficiency of the ScAM strains dropped significantly when the environment did not contain glucose as a primary carbon source, indicating that mating is an energetically expensive process and growth on alternate carbon sources qualitatively reduces the efficiency of mating. When allowed to mate for different time periods, no mating was observed for the first four to five hours.The first diploids appeared only after four to five hours, indicating that this is the time needed for the mating process to be completed in the given environment. After that, the mating efficiency increased rapidly. Beyond seven hours, mating efficiency calculations were influenced by the fact that mitotic growth started on the plate.Ensure that equal number of both haploids are mixed. Deviations from this would lead to variations in independent repeats of the protocol. How gene flow impacts adaptation and speciation is an open question.Since the auxotrophic markers are inserted next to the MAT locus in our strains, this helps address this important question.

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JoVE Journal

Biology

2.2K visualizações.  Indian Institute of Technology Bombay. Este protocolo é um método simples para quantificar a eficiência com que diferentes cepas e espécies de leveduras se acasalam entre si. O método proposto é simples, robusto e eficiente. O método pode ser estendido a qualquer cepa de levedura.O método descrito neste estudo pode ser usado para entender como ocorre a especiação em leveduras. Além disso, as cepas que descrevemos podem ser particularmente úteis para projetar experimentos com fluxo gênico. A força do método é...