Patching of Haploids in the Mating Efficiency Grids
1:55
Mixing of Haploids and Mating
2:55
Plating of Mixed Cells on YPD Agar and Screening for Diploids using Auxotrophic Markers
3:53
Results: Quantification and Dynamics of Mating Efficiency in Saccharomyces cerevisiae (Yeast)
5:03
Conclusion
필기록
This protocol is a simple method to quantify the efficiency with which different strains and species of yeast mate with each other. The proposed method is simple, robust, and efficient. The method can be extended to any strain of yeast.
The
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In this work, a robust method for the quantification of mating efficiency in the yeast Saccharomyces cerevisiae is described. This method is particularly useful for the quantification of pre-zygotic barriers in speciation studies.