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JoVE Journal

Biochemistry

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Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

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06:39 min

June 30th, 2023

June 30th, 2023

695 Views

0:00

Introduction

0:44

Flow Cytometry Setting and Preparation of Autoclaved Spores for Flow Cytometry

1:47

Analysis Using Flow Cytometry

3:27

Estimation of the Protein Coupling Index on a Spore Surface Using Flow Cytometry

4:52

Results: Fluorescence Coupling Percentage Analysis of Bacillus subtilis Spores

6:08

Conclusion

Transcrição

Cell counter of Bacillus subtilis spores using traditional methods can be a laborious task, as these methods can be completely manual and their accuracies depending on the operator's experience. This protocol makes the enumeration of dormant and g

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This protocol focuses on the use of flow cytometry and counting beads to quantify bacterial spores labeled with ethidium bromide. The method is also efficient for analyzing the covalent coupling of proteins on the surface of intact spores.

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