Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

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06:39 min

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June 30th, 2023

DOI :

10.3791/65141-v

June 30th, 2023

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Késsia Caroline Alves¹^,², Yury Oliveira Chaves¹^,³^,⁴, Maria Edilene Almeida¹^,⁴, Maria Gabriella Vasconcelos¹^,⁴, Paulo Afonso Nogueira¹^,³^,⁶, Joyce Melo¹^,⁹, Jéssica Marques⁸, Juliana Pavan Zuliani⁷, Charles Nunes Boeno⁷, Mauro Valentino Paloschi⁷, Rachele Isticato⁵, Ezio Ricca⁵, Luís André Mariúba¹^,²^,³^,⁶

¹Laboratório de diagnóstico e controle de doenças infecciosas na Amazônia, Instituto Leônidas e Maria Deane - Fiocruz Amazônia, ²Programa de Pós-Graduação em Biotecnologia, Instituto de Ciências Biológicas, Universidade Federal do Amazonas (UFAM), ³Programa de Pós-Graduação em Biologia da Interação Patógeno-Hospedeiro - Fiocruz Amazônia, ⁴Programa de Pós-graduação em Ciências Aplicadas à Hematologia PPGH-UEA/HEMOAM, ⁵Department of Biology, Federico II University, ⁶Programa de Pós-Graduação em Imunologia Básica e Aplicada, Instituto de Ciências Biológicas, Universidade Federal do Amazonas (UFAM), ⁷Laboratório de Imunologia Celular Aplicada à saúde, Fundação Oswaldo Cruz, FIOCRUZ Rondônia, ⁸Centro Multiusuário para Análises de Fenômenos Biomédicos (CMABio-UEA), Universidade Estadual do Amazonas, ⁹Universidade Federal do Amazonas (UFAM)

Summary

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Fluorescence Characteristics

Acquisition Parameters

Fluorescent Proteins

Chapters in this video

0:00

Introduction

0:44

Flow Cytometry Setting and Preparation of Autoclaved Spores for Flow Cytometry

1:47

Analysis Using Flow Cytometry

3:27

Estimation of the Protein Coupling Index on a Spore Surface Using Flow Cytometry

4:52

Results: Fluorescence Coupling Percentage Analysis of Bacillus subtilis Spores

6:08

Conclusion

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