Development of pHluorin2-Based Sensor for Glycosomal pH in Trypanosoma brucei

January 19th 2024


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We describe a method to study how pH responds to environmental cues in the glycosomes of the bloodstream form of African trypanosomes. This approach involves a pH-sensitive heritable protein sensor in combination with flow cytometry to measure pH dynamics, both as a time-course assay and in a high-throughput screen format.

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