Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

Summary

The goal of this protocol is to use cationic/anionic liposomes with a neuro-targeting peptide as a CNS delivery system to enable siRNA to cross the BBB. The optimization of a delivery system for treatments, like siRNA, would allow for more treatment options for prion and other neurodegenerative diseases.

Abstract

Prion diseases result from the misfolding of the normal, cellular prion protein (PrP^C) to an abnormal protease resistant isomer called PrP^Res. The emergence of prion diseases in wildlife populations and their increasing threat to human health has led to increased efforts to find a treatment for these diseases. Recent studies have found numerous anti-prion compounds that can either inhibit the infectious PrP^Res isomer or down regulate the normal cellular prion protein. However, most of these compounds do not cross the blood brain barrier to effectively inhibit PrP^Res formation in brain tissue, do not specifically target neuronal PrP^C, and are often too toxic to use in animal or human subjects.

We investigated whether siRNA delivered intravascularly and targeted towards neuronal PrP^C is a safer and more effective anti-prion compound. This report outlines a protocol to produce two siRNA liposomal delivery vehicles, and to package and deliver PrP siRNA to neuronal cells. The two liposomal delivery vehicles are 1) complexed-siRNA liposome formulation using cationic liposomes (LSPCs), and 2) encapsulated-siRNA liposome formulation using cationic or anionic liposomes (PALETS). For the LSPCs, negatively charged siRNA is electrostatically bound to the cationic liposome. A positively charged peptide (RVG-9r [rabies virus glycoprotein]) is added to the complex, which specifically targets the liposome-siRNA-peptide complexes (LSPCs) across the blood brain barrier (BBB) to acetylcholine expressing neurons in the central nervous system (CNS). For the PALETS (peptide addressed liposome encapsulated therapeutic siRNA), the cationic and anionic lipids were rehydrated by the PrP siRNA. This procedure results in encapsulation of the siRNA within the cationic or anionic liposomes. Again, the RVG-9r neuropeptide was bound to the liposomes to target the siRNA/liposome complexes to the CNS. Using these formulations, we have successfully delivered PrP siRNA to AchR-expressing neurons, and decreased the PrP^C expression of neurons in the CNS.

Introduction

Prions are severe neurodegenerative diseases that affect the CNS. Prion diseases result from the misfolding of the normal cellular prion protein, PrP^C, by an infectious isomer called PrP^Res. These diseases affect a wide variety of species including bovine spongiform encephalopathy in cows, scrapie in sheep, chronic wasting disease in cervids, and Creutzfeldt-Jakob disease in humans^1-3. Prions cause neurodegeneration that starts with synaptic loss, and progresses to vacuolization, gliosis, neuronal loss, and plaque deposits. Eventually, resulting in the death of the animal/individual⁴. For decades, researchers have investigated compounds meant to slow or stop the progression of prion disease. However, researchers have not found either a successful therapy or an effective systemic delivery vehicle.

Endogenous PrP^C expression is required for the development of prion diseases⁵. Therefore, decreasing or eliminating PrP^C expression may result in a delay or amelioration of disease. Several groups created transgenic mice with reduced levels of PrP^C or injected lentivectors expressing shRNA directly into murine brain tissue to investigate the role of PrP^C expression levels in prion disease. These researchers found reducing the amount of neuronal PrP^C resulted in halting the progressive neuropathology of prion diseases and extended the life of the animals^6-9. We have reported that PrP^C siRNA treatment results in clearance of PrP^Res in mouse neuroblastoma cells¹⁰. These studies suggest that the use of therapies to decrease PrP^C expression levels, like small interfering RNA (siRNA), which cleaves mRNA, may sufficiently delay the progression of prion diseases. However, most therapies investigated for prion diseases were delivered in ways that would not be practical in a clinical setting. Therefore, a siRNA therapy needs a systemic delivery system, which is delivered intravenously and targeted to the CNS.

Investigators have studied the use of liposomes as delivery vehicles for gene therapy products. Cationic and anionic lipids are both used in the formation of liposomes. Cationic lipids are more widely used than anionic lipids because the charge difference between the cationic lipid and the DNA/RNA allows for efficient packaging. Another advantage of cationic lipids is that they cross the cell membrane more easily than other lipids^11-14. However, cationic lipids are more immunogenic than anionic lipids^13,14. Therefore, researchers have started to shift from using cationic to anionic lipids in liposomes. Gene therapy products can be efficiently packaged into anionic liposomes using the positively charged peptide protamine sulfate, which condenses DNA/RNA molecules^15-19. Since anionic lipids are less immunogenic than cationic lipids they may have increased circulation times, and may be more tolerated in animal models^13,14. Liposomes are targeted to specific tissues using targeting peptides that are attached to the liposomes. The RVG-9r neuropeptide, which binds to nicotinic acetylcholine receptors, has been used to target siRNA and liposomes to the CNS^17-20.

This report outlines a protocol to produce three siRNA delivery vehicles, and to package and deliver the siRNA to neuronal cells (Figure 1). Liposome-siRNA-peptide complexes (LSPCs) are composed of liposomes with siRNA and the RVG-9r targeting peptide electrostatically attached to the outer surface of the liposome. Peptide addressed liposome encapsulated therapeutic siRNA (PALETS) are composed of siRNA and protamine encapsulated within the liposome, with RVG-9r covalently bonded to lipid PEG groups. Using the below methods to generate LSPCs and PALETS, PrP^C siRNA decreases PrP^C expression up to 90% in neuronal cells, which holds tremendous promise to cure or substantially delay the onset of prion disease pathology.

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Protocol

All mice were bred and maintained at Lab Animal Resources, accredited by the Association for Assessment and Accreditation of Lab Animal Care International, in accordance with protocols approved by the Institutional Animal Care and Use Committee at Colorado State University.

1. Preparation of LSPCs

1. Use a 1:1 DOTAP (1,2-dioleoyl-3-trimethylammonium-propane):cholesterol ratio for LSPCs. For a 4 nmole liposome preparation, mix 2 nmoles of DOTAP and 2 nmoles of cholesterol into 10 ml of a 1:1 chloroform:methanol solution in a flask.
2. Evaporate 9 ml of the chloroform:methanol solution using N₂ gas in a fume hood. Evaporate the last 1 ml of solvent in a fume hood overnight without gas. Observe a thin lipid film on the bottom of the flask.
3. Heat 10 ml of a 10% sucrose solution to 55 °C.
4. Pour the heated 10% sucrose solution onto the thin lipid film slowly, i.e. 1 ml/min, while gently swirling the flask. Maintain the 10% sucrose and flask with lipids at 55 °C so that the lipid molecules remain in the gel-liquid phase. Rehydration results in multilamellar vesicle (MLV) formation.
5. Allow lipids to rehydrate at 55 °C for at least one hr before sizing the liposomes.
6. Assemble an extruder per manufacturer's instructions with 1.0 µm filters or use 1.0 µm syringe filters for sizing.
7. Add 1 ml of the heated liposome suspension to one of the syringes on the extruder, and pass the suspension between the two syringes 11 times. Make sure that the suspension is in the opposite syringe than the starting syringe after the 11 passes.
8. Size the liposomes again through 0.45 µm then 0.2 µm filters to generate large unilamellar vesicles (LUVs). Keep the liposome suspension heated for easier sizing.
9. Incubate 20 µl of the 4 nmole liposome suspension (200 µM) with 200 µl of a 20 µM (4 nmole total) stock siRNA solution for 10 min at room temperature.
10. Incubate 80 µl of a 500 µM (40 nmole total) stock RVG-9r solution with the siRNA/liposome solution for 10 min at RT. LSPCs should be used as soon as possible but can be used up to several hours after preparation. Store LSPCs at 4 °C for several hr after preparation.

2. Preparation of PALETS

1. Use a 55:40:5 molar ratio of either DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine):cholesterol:DSPE-PEG or DOTAP:cholesterol:DSPE-PEG for PALETS. For a 4 nmole liposome preparation, mix 2.2 nmoles of either DSPE or DOTAP, 1.6 nmoles of cholesterol, and 0.2 nmoles of DSPE-PEG into 10 ml of a 2:1 chloroform:methanol solution in a flask.
2. Prepare liposome suspension exactly as described in Step 1.1 and 1.2 above. Rehydrate DSPE liposomes in 10 ml of 1x PBS heated to 75 °C, adding 1 ml/min. Allow lipids to rehydrate at 75 °C for at least 1 hr before sizing.
3. Size the liposome suspension exactly as described in Step 1.6-1.8.
4. Pipet 20 µl of each of the 4 nmole (200 µM) liposome suspensions into single use aliquots after DOTAP and DSPE liposomes have been sized, and lyophilize for 30 min using a benchtop freeze dryer (Figure 2).
5. Incubate 200 µl of a 20 µM (4 nmole total) stock siRNA solution with 1.4 µl of a 26.6 µM solution of protamine sulfate for 10 min at RT for siRNA that is to be encapsulated into DSPE PALETS liposomes.
6. Rehydrate the DOTAP PALETS liposomes with 200 µl of a 4 nmole stock solution of siRNA or rehydrate DSPE PALETS liposomes with 201.4 µl of the siRNA/protamine solution. Incubate liposome/siRNA solution for 10 min at RT.
7. Add 10 µl of a 60 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) solution to the liposome/siRNA solution for both DOTAP and DSPE PALETS.
8. Add 10 µl of a 150 mM N-hydroxysulfosuccinimide (sulfo-NHS) solution to the liposome/siRNA solution for both DOTAP and DSPE PALETS.
9. Incubate the EDC and sulfo-NHS with the siRNA/liposome suspension for 2 hr at RT.
10. Incubate 80 µl of a 500 µM (40 nmole total) stock RVG-9r solution with the siRNA/liposome crosslinking solution for 10 min at RT.
    NOTE: The EDC/sulfo-NHS allows the RVG-9r to covalently bond to the PEG lipids. Use PALETS within several hr after preparation. Store PALETS at 4 °C for several hr after preparation.
11. To determine siRNA encapsulation efficiency of PALETS:
    1. Measure the concentration of siRNA before and after the addition of protamine and liposomes using a spectrophotometer set at 260 nm.
    2. Filter the unencapsulated siRNA from the liposome/encapsulated siRNA suspension using 50 kDa centrifugal filters. Centrifuge at 1,000 x g for 20 min.
    3. Measure the concentration of unencapsulated siRNA in the filtrate and the concentration of encapsulated siRNA in the retentate using a spectrophotometer at 260 nm.

3. Injecting Mice with LSPCs or PALETS

1. Expose mice for 5-10 min to a heat lamp to dilate vasculature.
2. Anesthetize mouse with a 2-3% isofluorane/oxygen flow 5-10 min before injection, and during injection. Confirm anesthetization when animal is no longer ambulant and respirations have slowed by doing a toe pinch. Place the mouse on its back or side to access the tail vein. Use vet ointment on mouse's eyes to prevent drying while under anesthesia.
3. Wipe/spray the tail with 70% EtOH and inject 300 µl of LSPCs or PALETS onto the tail vein of the mouse using a 26 G insulin syringe. Start injecting distally into the tail vein and move proximally if the vein collapses or ruptures.
4. Place mouse in a clean cage until it maintains sternal recumbency. Do not place mouse with other cage mates until it has fully recovered. Mice should recover in 5 to 15 min.  They can be placed on a heating pad or under a heat lamp to maintain body temperature if recovery takes longer.  Mice should be monitored closely to safeguard against over-heating.
5. Monitor the animal several hours after injection to ensure anesthesia wears off, and there are no ill effects of the treatment. Allow the LSPCs or PALETS to circulate for at least 24 hr.

4. Analysis of Protein Expression via Flow Cytometry

1. Euthanize mice with a 20% CO₂ flow rate for 15 min.
2. Dissect out half a hemisphere of brain by cutting away the skin and skull of the mouse using scissors. Peel away half a hemisphere of brain away from the skull using tongs or the end of a pair of scissors.
3. Press half a hemisphere of brain from each mouse through a 40 µm mesh cell strainer using 2.5 ml of FACS buffer (1x PBS, 1% fetal bovine serum, 10 mM EDTA) in a Petri dish with the plunger of a syringe.
4. Rinse the cell strainer and Petri dish with an additional 2.5 ml of FACS buffer. Put the single cell suspension in a 15 ml conical tube on ice.
5. Pipet 100 µl of the 5 ml single cell suspension into a 1.5 ml microcentrifuge tube and centrifuge for 5 min at 350 x g. Discard the supernatant and resuspend cell pellet in 1 ml of FACS buffer. Spin at 350 x g for 5 min. Repeat with another 1 ml of FACS buffer. Keep the cell suspension on ice.
6. Incubate the cells in 100 µl of 1:100 dilution of a 0.5 mg/ml rat anti-mouse Fc block in FACS buffer for 30-60 min on ice. Pellet and wash the cells as in step 4.5. Keep the cell suspension on ice.
7. Incubate the cells in 100 µl of a 20 µg/ml fluorescent antibody against mouse PrP^C in 7% mouse serum in FACS buffer on ice for 30-60 min. Pellet and wash the cells as in step 4.5. Keep the cell suspension on ice.
8. Pellet and re-suspend the cells in 1 ml of RBC lysis buffer (1x PBS, 155 mM NH₄Cl, 12 mM NaHCO₃, 0.1 mM EDTA) for 1 min. Pellet as in step 4.5 and resuspend the cells in 1 ml of FACS buffer. Keep the cell suspension on ice.
9. Analyze PrP^C expression using a flow cytometer as described previously¹⁰. Gate live cells from total cell population. Gate PrP^C + cells from live cell population to determine MFI (median fluorescent intensity).

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Results

To increase the efficiency of siRNA encapsulation within anionic PALETS, the siRNA was mixed with protamine. To determine the best protamine concentration for the siRNA, the siRNA was mixed with different concentrations of protamine, from 1:1 to 2:1 (Figure 3A). There was a 60-65% siRNA encapsulation efficiency in anionic liposomes without the use of protamine. Samples with protamine:siRNA molar ratios from 1:1 to 1.5:1 (133-266 nM) had 80-90% siRNA encapsulation. Molar r...

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Discussion

This report describes a protocol to create two targeted delivery systems that efficiently transports siRNA to the CNS. Previous methods of delivering siRNA to the CNS included injecting siRNA/shRNA vectors directly into the brain, intravenous injection of targeted siRNA, or intravenous injection of non-targeted liposome-siRNA complexes. Injection of siRNA/shRNA vectors into the CNS does cause a decrease in target protein expression levels. However, the siRNA/shRNA does not diffuse freely through the CNS. Furthermore, the...

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Materials

Name	Company	Catalog Number	Comments
DOTAP lipid	Avanti Lipids	890890
Cholesterol	Avanti Lipids	700000
DSPE	Avanti Lipids	850715
DSPE-PEG	Avanti Lipids	880125
Chloroform	Fisher Scientific	AC268320010
Methanol	EMD Millipore	113351
N2 Gas	AirGas
Sucrose	Fisher Scientific	S5-500
Extruder	Avanti Lipids	610023
1.0, 0.4, and 0.2 μm filters	Avanti Lipids	610010, 610007, 610006
PBS	Life Technologies	70011-044
Protamine sulfate	Fisher Scientific	ICN10275205
EDC	Thermo Scientific	22980	Aliquoted for single use
Sulfo-NHS	Thermo Scientific	24510	Aliquoted for single use
40 μm Cell Strainer	Fisher Scientific	08-771-1
Rat anti-mouse CD16/CD32 Fc block	BD Pharmigen	553141
Anti-PrP antibody (PRC5)	Proprietary - PRC