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Abstract

Neuroscience

Laser-Induced Brain Injury in the Motor Cortex of Rats

Published: September 26th, 2020

DOI:

10.3791/60928

1Division of Anesthesiology and Critical Care, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, 2Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, 3Department of Biochemistry and Physiology, Faculty of Biology, Ecology, and Medicine, Oles Honchar Dnipro National University, 4Department of Anesthesiology, Yale University School of Medicine, 5Department of Ophthalmology, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev

* These authors contributed equally

ERRATUM NOTICE

Important: There has been an erratum issued for this article. Read more …

Abstract

A common technique for inducing stroke in experimental rodent models involves the transient (often denoted as MCAO-t) or permanent (designated as MCAO-p) occlusion of the middle cerebral artery (MCA) using a catheter. This generally accepted technique, however, has some limitations, thereby limiting its extensive use. Stroke induction by this method is often characterized by high variability in the localization and size of the ischemic area, periodical occurrences of hemorrhage, and high death rates. Also, the successful completion of any of the transient or permanent procedures requires expertise and often lasts for about 30 minutes. In this protocol, a laser irradiation technique is presented that can serve as an alternative method for inducing and studying brain injury in rodent models.

When compared to rats in the control and MCAO groups, the brain injury by laser induction showed reduced variability in body temperature, infarct volume, brain edema, intracranial hemorrhage, and mortality. Furthermore, the use of a laser-induced injury caused damage to the brain tissues only in the motor cortex unlike in the MCAO experiments where destruction of both the motor cortex and striatal tissues is observed.

Findings from this investigation suggest that laser irradiation could serve as an alternative and effective technique for inducing brain injury in the motor cortex. The method also shortens the time for completing the procedure and does not require expert handlers.

Erratum

Erratum: Laser-Induced Brain Injury in the Motor Cortex of Rats

An erratum was issued for: Laser-Induced Brain Injury in the Motor Cortex of Rats. The Authors section was updated.

One of the author names was updated from:

Dmitri Frank

to

Dmitry Frank

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Keywords Laser induced Brain Injury

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