Sign In

A subscription to JoVE is required to view this content. Sign in or start your free trial.

In This Article

  • Summary
  • Abstract
  • Introduction
  • Protocol
  • Representative Results
  • Discussion
  • Acknowledgements
  • Materials
  • References
  • Reprints and Permissions

Summary

A high platform can fix rats without restriction and completely expose the acupoints on the back during acupuncture manipulation. This article describes methods for the fabrication of the high platform, establishes a rat model of asthma and measures changes in respiratory function using a noninvasive and real-time whole-body plethysmography (WBP) system.

Abstract

A high platform can fix rats without restriction and completely expose the acupoints on the back during acupuncture manipulation. This article describes methods for the fabrication of the high platform, establishes a rat model of asthma and measures changes in respiratory function using a noninvasive and real-time whole-body plethysmography (WBP) system.

Introduction

Asthma is a chronic disease characterized by bronchoconstriction and airway hyper-responsiveness1. Approximately 339.4 million people worldwide are diagnosed with asthma2. The prevalence of asthma is increasing globally, with 100 million new cases expected in the next decade3. Inhaled corticosteroids and long-acting β2-agonists do not effectively control the symptoms of all patients4. Therefore, the use of alternative therapies such as acupuncture to treat asthma has been receiving increased attention5. An effective method for treatin....

Protocol

All animal experiments were reviewed and approved by the Committee on the Ethics of Animal Experiments of Shanghai University of Traditional Chinese Medicine (Ethical clearance number PZSHUTCM190308020).

1. Fabrication of the high platform

  1. Set the length and width of the supporting device to 86 cm x 17 cm (Figure 1).
  2. Fix the connecting device vertically to the supporting device. The connecting device should b.......

Representative Results

With acupuncture at the GV14, bilateral BL12 and bilateral BL13 points, the rat maintains a prone position. The rat can twist its head only to the outside and not the inside because one direction is blocked with a wooden board (Figure 5).

As the methacholine concentration increased, Penh shows an increasing trend and the growth rate gradually increases. At concentrations of 3.125 mg/mL, 6.25 mg/mL, 12.5 mg/mL, 25 mg/mL and 50 mg/mL, Penh increased by 18.40% ±.......

Discussion

The GV14, bilateral BL12 and bilateral BL13 acupoints used in this study are located on the back, and thus the high platform is suitable for fixing rats. In other studies, an adequate fixation method should be selected according to the position of the acupoints15. For acupoints on the abdomen, the rat can be placed downward in a black cone (similar to a pastry bag used by bakers)17. A chain is attached to the restraint cone for fixation, and the hind leg of the rat is also .......

Acknowledgements

This work was supported by National Natural Science Foundation of China (No. 81922076, 81973951, 81873373), Construction project of collaborative innovation of shanghai (No. ZYJKFW201811010, ZYJKFW201701005).

....

Materials

NameCompanyCatalog NumberComments
0.3 mm x 13.0 mm acupuncture needleChina Suzhou Medical Device Factory
Al(OH)3American Thermo77161
Compression nebulizerJiangsu Lude Medical Electronics Co., Ltd.NB-212C
Fine Pointe V2.0American Buxco
Laboratory gas drying unitAmerican Drierite26800
MethacholineAmerican SigmaMKCF6054
OvalbuminAmerican SigmaA5503-25G
Phosphate Buffer solution (1x)GelifesciencesSH30256.01
Seal boxIRIS Corporation of Japan
Whole-body plethysmography respiratory function measurement systemAmerican Buxco

References

  1. Papi, A., Brightling, C., Pedersen, S. E., Reddel, H. K. Asthma. Lancet. 391 (10122), 783-800 (2018).
  2. . Global strategy for asthma management and prevention Available from: https://ginasthma.org/gina-reports/ (2019)
  3. Enilari, O., Sinha, S.

Explore More Articles

AcupunctureAsthmaRat ModelAnimal StudyOVA SensitizationNebulizationAcupuncture PointsGV 14BL 12BL 13Manual AcupunctureTraditional Chinese Medicine

This article has been published

Video Coming Soon

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved