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Abstract

Developmental Biology

Intramyocardial Injection for the Study of Cardiac Lymphatic Function in Zebrafish

Published: September 20th, 2022

DOI:

10.3791/64504

1Cardiovascular Research Institute, Weill Cornell Medicine, 2Department of Cell and Developmental Biology, Weill Cornell Medicine, 3The Saban Research Institute and Heart Institute, Children's Hospital Los Angeles

* These authors contributed equally

Abstract

Zebrafish have proved to be an important model for studying cardiovascular formation and function during postembryonic development and regeneration. The present protocol describes a method for injecting fluorescent tracers into the zebrafish myocardium to study interstitial fluid and debris uptake into cardiac lymphatic vessels. To do so, microspheres (200 nm diameter) and quantum dots (<10 nm diameter) are introduced into the myocardium of live zebrafish, which can be tracked using ex vivo confocal microscopy. These tracers are then tracked intermittently over several hours to follow clearance from the myocardium into cardiac lymphatic vessels. Quantum dots are transported through cardiac lymphatic vessels away from the heart, while larger microspheres remain at the injection site for over three weeks. This method of intramyocardial injection can be extended to other uses, including the injection of encapsulated MS or hydrogels to locally release cells, proteins, or compounds of interest to a targeted region of the heart.

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Keywords Intramyocardial Injection

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