A subscription to JoVE is required to view this content. Sign in or start your free trial.
This protocol details the utilization of a polyol-based microwave-assisted extraction method for extracting phenolic compounds and natural antioxidants, representing a practical and environmentally sustainable approach to the development of ready-to-use extracts.
The utilization of polyols as green solvents for extracting bioactive compounds from plant materials has gained attention due to their safety and inert behavior with plant bioactive chemicals. This study explores the sustainable extraction of phenolic compounds and natural antioxidants from coffee silverskin using the microwave-assisted extraction (MAE) method with polyol-based solvents: glycerin, propylene glycol (PG), butylene glycol (BG), methylpropanediol (MPD), isopentyldiol (IPD), pentylene glycol, 1,2-hexanediol, and hexylene glycol (HG). A comparative analysis was conducted on conventional and non-conventional solvent extractions, focusing on their impact on the bioactive compounds of MAE, encompassing parameters such as total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities like the 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay (DPPH), the 2,2′-azino-bis(-3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging assay (ABTS), and the ferric reducing antioxidant power assay (FRAP). The highest values were observed for TPC with aqueous-1,2-hexanediol extraction (52.0 ± 3.0 mg GAE/g sample), TFC with aqueous-1,2-hexanediol extraction (20.0 ± 1.7 mg QE/g sample), DPPH with aqueous-HG extraction (13.6 ± 0.3 mg TE/g sample), ABTS with aqueous-pentylene glycol extraction (8.2 ± 0.1 mg TE/g sample), and FRAP with aqueous-HG extraction (21.1 ± 1.3 mg Fe (II) E/g sample). This research aims to advance eco-friendly extraction technology through natural plant components, promoting sustainability by minimizing hazardous chemical use while reducing time and energy consumption, with potential applications in cosmetics.
Nowadays, there is a global trend towards environmental awareness in the beauty industry, leading manufacturers to focus on green technology for extracting plant components using sustainable alternatives1. Typically, traditional solvents such as ethanol, methanol, and hexane are used to extract plant phenolic components and natural antioxidants2. Nevertheless, the presence of solvent residues within plant extracts poses a potential risk to human health, inducing skin and eye irritation3, particularly concerning their intended application in cosmetics. Consequently, it is challenging to eliminate s....
The details of the reagents and the equipment used in this study are listed in the Table of Materials.
1. Experimental preparation
Effect of polyols solvents and conventional solvents on total phenolic content, total flavonoid content, DPPH, FRAP, and ABTS antioxidant assays
Solvent polarity should be compatible with that of targeted active molecules to improve the extraction efficiency of bioactive substances from plants22. Experiments were conducted using various solvents (water, ethanol, glycerin, propylene glycol, butylene glycol, methylpropanediol, isopentyldiol, pentylene glycol, 1,2-hexanediol, a.......
Various factors play a crucial role in the successful implementation of MAE, such as the phytochemical content of plant components, extraction duration, temperature, microwave power, solid-liquid ratio, and solvent concentration13. Plants typically exhibit varying profiles of phytochemicals; hence, the selection of natural plants rich in antioxidants and phenolic compounds is essential23. Furthermore, distinct bioactive constituents display a variety of polarities depending.......
This study was funded by Mae Fah Luang University. The authors would like to acknowledge the Tea and Coffee Institute of Mae Fah Luang University for facilitating the connection between the researchers and local farmers concerning the acquisition of coffee silverskin samples.
....Name | Company | Catalog Number | Comments |
1,2-Hexanediol | Chanjao Longevity Co., Ltd. | ||
2,2 -Azino-bis 3 ethylbenzothiazoline-6-sulfonic acid diammonium salt (ABTS) | Sigma | A1888 | |
2,2-Diphenyl-1-picrylhydrazyl (DPPH) | Sigma | D9132 | |
2,4,6-Tri(2-pyridyl)-s-triazine (TPTZ) | Sigma | 93285 | |
2-Digital balance | Ohaus | Pioneer | |
4-Digital balance | Denver | SI-234 | |
6-hydroxy-2,5,7,8 tetramethylchroman -2-carboxylic acid (Trolox) | Sigma | 238813 | |
96-well plate | SPL Life Science | ||
Absolute ethanol | RCI Labscan | 64175 | |
Acetic acid | RCI Labscan | 64197 | |
Aluminum chloride | Loba Chemie | 898 | |
Automatic pipette | Labnet | Biopett | |
Butylene glycol | Chanjao Longevity Co., Ltd. | ||
Ethos X advanced microwave extraction | Milestone Srl, Sorisole, Italy | ||
Ferrous sulfate | Ajex Finechem | 3850 | |
Folin-Ciocalteu's reagent | Loba Chemie | 3870 | |
Freezer SF | Sanyo | C697(GYN) | |
Gallic acid | Sigma | 398225 | |
Grinder | Ou Hardware Products Co.,Ltd | ||
Hexylene glycol | Chanjao Longevity Co., Ltd. | ||
Hydrochloric acid (37%) | RCI Labscan | AR1107 | |
Iron (III) chloride | Loba Chemie | 3820 | |
Isopentyldiol | Chanjao Longevity Co., Ltd. | ||
Methanol | RCI Labscan | 67561 | |
Methylpropanediol | Chanjao Longevity Co., Ltd. | ||
Pentylene glycol | Chanjao Longevity Co., Ltd. | ||
Potassium persulfate | Loba Chemie | 5420 | |
Propylene glycol | Chanjao Longevity Co., Ltd. | ||
Quercetin | Sigma | Q4951 | |
Refrigerated centrifuge | Hettich | ||
Sodium acetate | Loba Chemie | 5758 | |
Sodium carbonate | Loba Chemie | 5810 | |
Sodium hydroxide | RCI Labscan | AR1325 | |
Sodium nitrite | Loba Chemie | 5954 | |
SPECTROstar Nano microplate reader | BMG- LABTECH | ||
SPSS software | IBM SPSS Statistics 20 | ||
Tray dryer | France Etuves | XUE343 |
Explore More Articles
This article has been published
Video Coming Soon
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved